BMRB Entry 27016

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27016

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the DHDD Region of GbnD4 KS14 from the Gladiolin Polyketide Synthase
Deposition date:
2017-01-27
Original release date:
2017-12-12
Authors:
Jenner, Matthew; Kosol, Simone; Lewandowski, Jozef; Challis, Gregory
Citation:

Citation: Jenner, Matthew; Kosol, Simone; Griffiths, Daniel; Prasongpolchai, Panward; Manzi, Lucio; Barrow, Andrew; Moses, John; Oldham, Neil; Lewandowski, Jozef; Challis, Gregory. "Mechanism of intersubunit ketosynthase-dehydratase interaction in polyketide synthases"  Nat. Chem. Biol. 14, 270-275 (2018).
PubMed: 29309054

Assembly members:

Assembly members:
DHDD, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Burkholderia gladioli   Taxonomy ID: 28095   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia gladioli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DHDD: MKHHHHHHHHGGLVPRGSHG SDEGVPDALRADTVPRAGPV RYARRRYWIGEARSDALAPA APLEREPLPAEAMGAYFAIR RTDADDTVAAH

Data sets:
Data typeCount
13C chemical shifts230
15N chemical shifts69
1H chemical shifts69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Protein DHDD1

Entities:

Entity 1, Protein DHDD 91 residues - Formula weight is not available

1   METLYSHISHISHISHISHISHISHISHIS
2   GLYGLYLEUVALPROARGGLYSERHISGLY
3   SERASPGLUGLYVALPROASPALALEUARG
4   ALAASPTHRVALPROARGALAGLYPROVAL
5   ARGTYRALAARGARGARGTYRTRPILEGLY
6   GLUALAARGSERASPALALEUALAPROALA
7   ALAPROLEUGLUARGGLUPROLEUPROALA
8   GLUALAMETGLYALATYRPHEALAILEARG
9   ARGTHRASPALAASPASPTHRVALALAALA
10   HIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks