BMRB Entry 27025
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27025
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Citation: Baronti, Lorenzo; Hosek, Tomas; Gil-Caballero, Sergio; Raveh-Amit, Hadas; Calcada, Eduardo; Ayala, Isabel; Dinnyes, Andras; Felli, Isabella; Pierattelli, Roberta; Brutscher, Bernhard. "Fragment-Based NMR Study of the Conformational Dynamics in the bHLH Transcription Factor Ascl1'" Biophys. J. 112, 1366-1373 (2017).
PubMed: 28402879
Assembly members:
Ascl1_fragment_C, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
Ascl1_fragment_C: MASHHHHHHQQQPAAVARRN
ERERNRVKLVNLGFATLREH
VPNGAANKKMSKVETLRSAV
EYIRALQQLLDEHDAVSAA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 208 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 67 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ascl1 trascription factor, Fragment C | 1 |
Entities:
Entity 1, Ascl1 trascription factor, Fragment C 79 residues - Formula weight is not available
Residues 1-9 represent the artificial affinity tag. Ascl1 fragment C chemical shift assignment start from residue 10 (residue 1 in the assignment file).
| 1 | MET | ALA | SER | HIS | HIS | HIS | HIS | HIS | HIS | GLN | ||||
| 2 | GLN | GLN | PRO | ALA | ALA | VAL | ALA | ARG | ARG | ASN | ||||
| 3 | GLU | ARG | GLU | ARG | ASN | ARG | VAL | LYS | LEU | VAL | ||||
| 4 | ASN | LEU | GLY | PHE | ALA | THR | LEU | ARG | GLU | HIS | ||||
| 5 | VAL | PRO | ASN | GLY | ALA | ALA | ASN | LYS | LYS | MET | ||||
| 6 | SER | LYS | VAL | GLU | THR | LEU | ARG | SER | ALA | VAL | ||||
| 7 | GLU | TYR | ILE | ARG | ALA | LEU | GLN | GLN | LEU | LEU | ||||
| 8 | ASP | GLU | HIS | ASP | ALA | VAL | SER | ALA | ALA |
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