BMRB Entry 27037

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27037

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Title:
Alanine chemical shifts of the N-terminal domain (residues 1-215) of HtpG, the Hsp90 from Escherichia coli. Northeast Structural Genomics Consortium Target ER697A.
Deposition date:
2017-02-24
Original release date:
2017-06-30
Authors:
Ramelot, Theresa; Pederson, Kari; Wang, Huang; Maglaqui, Melissa; Mao, Lei; Xiao, Rong; Acton, Thomas; Everett, John; Prestegard, James; Montelione, Gaetano; Kennedy, Michael
Citation:

Citation: Pederson, Kari; Chalmers, Gordon; Gao, Qi; Elnatan, Daniel; Ramelot, Theresa; Ma, Li-Chung; Montelione, Gaetano; Kennedy, Michael; Agard, David; Prestegard, James. "NMR characterization of HtpG, the E. coli Hsp90, using sparse labeling with (13)C-methyl alanine"  J. Biomol. NMR 68, 225-236 (2017).
PubMed: 28653216

Assembly members:

Assembly members:
NTD, polymer, 214 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NTD: KGQETRGFQSEVKQLLHLMI HSLYSNKEIFLRELISNASD AADKLRFRALSNPDLYEGDG ELRVRVSFDKDKRTLTISDN GVGMTRDEVIDHLGTIAKSG TKSFLESLGSDQAKDSQLIG QFGVGFYSAFIVADKVTVRT RAAGEKPENGVFWESAGEGE YTVADITKEDRGTEITLHLR EGEDEFLDDWRVRSIISKYS DHIALPVEIEKREE

Data sets:
Data typeCount
13C chemical shifts39
15N chemical shifts13
1H chemical shifts65

Time Domain Data

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1NTD1

Entities:

Entity 1, NTD 214 residues - Formula weight is not available

10 N-terminal residues not included: MGHHHHHHSH

1   LYSGLYGLNGLUTHRARGGLYPHEGLNSER
2   GLUVALLYSGLNLEULEUHISLEUMETILE
3   HISSERLEUTYRSERASNLYSGLUILEPHE
4   LEUARGGLULEUILESERASNALASERASP
5   ALAALAASPLYSLEUARGPHEARGALALEU
6   SERASNPROASPLEUTYRGLUGLYASPGLY
7   GLULEUARGVALARGVALSERPHEASPLYS
8   ASPLYSARGTHRLEUTHRILESERASPASN
9   GLYVALGLYMETTHRARGASPGLUVALILE
10   ASPHISLEUGLYTHRILEALALYSSERGLY
11   THRLYSSERPHELEUGLUSERLEUGLYSER
12   ASPGLNALALYSASPSERGLNLEUILEGLY
13   GLNPHEGLYVALGLYPHETYRSERALAPHE
14   ILEVALALAASPLYSVALTHRVALARGTHR
15   ARGALAALAGLYGLULYSPROGLUASNGLY
16   VALPHETRPGLUSERALAGLYGLUGLYGLU
17   TYRTHRVALALAASPILETHRLYSGLUASP
18   ARGGLYTHRGLUILETHRLEUHISLEUARG
19   GLUGLYGLUASPGLUPHELEUASPASPTRP
20   ARGVALARGSERILEILESERLYSTYRSER
21   ASPHISILEALALEUPROVALGLUILEGLU
22   LYSARGGLUGLU

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