BMRB Entry 27042
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27042
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Title: FHA-Chk2 PubMed: 32001251
Deposition date: 2017-03-05 Original release date: 2021-07-22
Authors: Hembram, Dambarudhar; Das, Ranabir; Negi, Hitendra; Shet, Divya
Citation: Hembram, Dambarudhar Shiba Sankar; Negi, Hitendra; Biswas, Poulomi; Tripathi, Vasvi; Bhushan, Lokesh; Shet, Divya; Kumar, Vikas; Das, Ranabir. "The Viral SUMO-Targeted Ubiquitin Ligase ICP0 is Phosphorylated and Activated by Host Kinase Chk2" J. Mol. Biol. 432, 1952-1977 (2020).
Assembly members:
FHA-Chk2, polymer, 162 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
FHA-Chk2: MHHHHHHLVPRGSETVSTQE
LYSIPEDQEPEDQEPEEPTP
APWARLWALQDGFANLECVN
DNYWFGRDKSCEYCFDEPLL
KRTDKYRTYSKKHFRIFREV
GPKNSYIAYIEDHSGNGTFV
NTELVGKGKRRPLNNNSEIA
LSLSRNKVFVFFDLTVDDQS
VY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 221 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 113 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FHA-Chk2 | 1 |
Entities:
Entity 1, FHA-Chk2 162 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | LEU | VAL | PRO | ||||
| 2 | ARG | GLY | SER | GLU | THR | VAL | SER | THR | GLN | GLU | ||||
| 3 | LEU | TYR | SER | ILE | PRO | GLU | ASP | GLN | GLU | PRO | ||||
| 4 | GLU | ASP | GLN | GLU | PRO | GLU | GLU | PRO | THR | PRO | ||||
| 5 | ALA | PRO | TRP | ALA | ARG | LEU | TRP | ALA | LEU | GLN | ||||
| 6 | ASP | GLY | PHE | ALA | ASN | LEU | GLU | CYS | VAL | ASN | ||||
| 7 | ASP | ASN | TYR | TRP | PHE | GLY | ARG | ASP | LYS | SER | ||||
| 8 | CYS | GLU | TYR | CYS | PHE | ASP | GLU | PRO | LEU | LEU | ||||
| 9 | LYS | ARG | THR | ASP | LYS | TYR | ARG | THR | TYR | SER | ||||
| 10 | LYS | LYS | HIS | PHE | ARG | ILE | PHE | ARG | GLU | VAL | ||||
| 11 | GLY | PRO | LYS | ASN | SER | TYR | ILE | ALA | TYR | ILE | ||||
| 12 | GLU | ASP | HIS | SER | GLY | ASN | GLY | THR | PHE | VAL | ||||
| 13 | ASN | THR | GLU | LEU | VAL | GLY | LYS | GLY | LYS | ARG | ||||
| 14 | ARG | PRO | LEU | ASN | ASN | ASN | SER | GLU | ILE | ALA | ||||
| 15 | LEU | SER | LEU | SER | ARG | ASN | LYS | VAL | PHE | VAL | ||||
| 16 | PHE | PHE | ASP | LEU | THR | VAL | ASP | ASP | GLN | SER | ||||
| 17 | VAL | TYR |
Samples:
sample_1: FHA-Chk2, [U-100% 13C; U-100% 15N], 200 uM; Phosphate 50 mM; NaCl 300 mM; DTT 2 mM
sample_conditions_1: ionic strength: 300 mM; pH: 7.4; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts