BMRB Entry 27056

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27056

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Title:
C. elegans SAS-5 N-term backbone assignments
Deposition date:
2017-03-24
Original release date:
2018-02-02
Authors:
Rogala, Kacper; Vakonakis, Ioannis
Citation:

Citation: Bianchi, Sarah; Rogala, Kacper; Dynes, Nicola; Hilbert, Manuel; Leidel, Sebastian; Steinmetz, Michel; Gonczy, Pierre; Vakonakis, Ioannis. "Interaction between the C. elegans centriolar protein SAS-5 and microtubules facilitates organelle assembly"  Mol. Biol. Cell 29, 722-735 (2018).
PubMed: 29367435

Assembly members:

Assembly members:
sas-5N, polymer, 123 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: C. elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pFLOAT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sas-5N: GPNNYDDLPCSIYFKKPTVQ EFVDQPRVFEDSEVPAFQEV LQLPQERTKPPVPSTQPIVA AVEVAKKKSCLSAPKPRKEP PSHPALRQKTVAFGKTVNVS QTVEGTSRNSKKVLASTMSA QNT

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts107
1H chemical shifts430

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1sas-5N1

Entities:

Entity 1, sas-5N 123 residues - Formula weight is not available

Residues 1 and 2 are cloning artifacts. Numbering in assignments corresponds to uniprot Q20010 except for the cloning artefact.

1   GLYPROASNASNTYRASPASPLEUPROCYS
2   SERILETYRPHELYSLYSPROTHRVALGLN
3   GLUPHEVALASPGLNPROARGVALPHEGLU
4   ASPSERGLUVALPROALAPHEGLNGLUVAL
5   LEUGLNLEUPROGLNGLUARGTHRLYSPRO
6   PROVALPROSERTHRGLNPROILEVALALA
7   ALAVALGLUVALALALYSLYSLYSSERCYS
8   LEUSERALAPROLYSPROARGLYSGLUPRO
9   PROSERHISPROALALEUARGGLNLYSTHR
10   VALALAPHEGLYLYSTHRVALASNVALSER
11   GLNTHRVALGLUGLYTHRSERARGASNSER
12   LYSLYSVALLEUALASERTHRMETSERALA
13   GLNASNTHR

Related Database Links:

UNP Q20010
AlphaFold G3MU16

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks