BMRB Entry 27079

Name: Backbone Resonance Assignment of the BCL6-BTB/POZ Domain
Published: 2017-10-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27079

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone Resonance Assignment of the BCL6-BTB/POZ Domain

Deposition date:

2017-04-20

Original release date:

2017-10-04

Authors:

Muskett, Frederick; Lin, Li-Ying; Wagner, Simon; Schwabe, John

Citation:

Citation: Lin, Li-Ying; Evans, S; Fairall, L; Schwabe, John; Wagner, Simon; Muskett, Frederick. "Backbone resonance assignment of the BCL6-BTB/POZ domain" Biomol. NMR Assignments 12, 47-50 (2018).
PubMed: 28929458

Assembly members:

Assembly members:
bl6, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bl6: GASQIQFTRHASDVLLNLNR LRSRDILTDVVIVVSREQFR AHKTVLMACSGLFYSIFTDQ LKRNLSVINLDPEINPEGFN ILLDFMYTSRLNLREGNIMA VMATAMYLQMEHVVDTCRKF IKAS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	228
15N chemical shifts	217
1H chemical shifts	215

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	bcl6	1

Entities:

Entity 1, bcl6 124 residues - Formula weight is not available

1

GLY

ALA

SER

GLN

ILE

GLN

PHE

THR

ARG

HIS

2

ALA

SER

ASP

VAL

LEU

ASN

LEU

ASN

ARG

3

LEU

ARG

SER

ARG

ASP

ILE

LEU

THR

ASP

VAL

4

VAL

ILE

VAL

SER

ARG

GLU

GLN

PHE

ARG

5

ALA

HIS

LYS

THR

VAL

LEU

MET

ALA

CYS

SER

6

GLY

LEU

PHE

TYR

SER

ILE

PHE

THR

ASP

GLN

7

LEU

LYS

ARG

ASN

LEU

SER

VAL

ILE

ASN

LEU

8

ASP

PRO

GLU

ILE

ASN

PRO

GLU

GLY

PHE

ASN

9

ILE

LEU

ASP

PHE

MET

TYR

THR

SER

ARG

10

LEU

ASN

LEU

ARG

GLU

GLY

ASN

ILE

MET

ALA

11

VAL

MET

ALA

THR

ALA

MET

TYR

LEU

GLN

MET

12

GLU

HIS

VAL

ASP

THR

CYS

ARG

LYS

PHE

13

ILE

LYS

ALA

SER

Samples:

sample_1: bl6, [U-100% 13C; U-100% 15N; U-80% 2H], 200 uM; sodium phosphate 50 mM; L-Arginine 50 mM; L-Glutamic acid 50 mM; TCEP 1 mM; sodium azide 3 mM

sample_2: bl6, [U-100% 13C; U-100% 15N; U-80% 2H], 200 uM; sodium phosphate 50 mM; TCEP 1 mM; sodium azide 3 mM; sodium chloride 300 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 400 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D HNCACB	sample_2	isotropic	sample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks