BMRB Entry 27109

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27109

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Title:
Solution-state NMR assignment of the flexible C-terminal domain of the human eye lens molecular chaperone alphaA-crystallin
Deposition date:
2017-05-18
Original release date:
2019-10-04
Authors:
Stavropoulou, Maria; Asami, Sam; Kaiser, Christoph; Haslbeck, Martin; Buchner, Johannes; Weinkauf, Sevil; Reif, Bernd
Citation:

Citation: Kaiser, Christoph; Peters, Carsten; Schmid, Philipp; Stavropoulou, Maria; Zou, Juan; Dahiya, Vinay; Mymrikov, Evgeny; Rockel, Beate; Asami, Sam; Haslbeck, Martin; Rappsilber, Juri; Reif, Bernd; Zacharias, Martin; Buchner, Johannes; Weinkauf, Sevil. "The structure and oxidation of the eye lens chaperone alphaA-crystallin"  Nat. Struct. Mol. Biol. 26, 1141-1150 (2019).
PubMed: 31792453

Assembly members:

Assembly members:
alphaA-crystallin_or_HspB1, polymer, 173 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alphaA-crystallin_or_HspB1: MDVTIQHPWFKRTLGPFYPS RLFDQFFGEGLFEYDLLPFL SSTISPYYRQSLFRTVLDSG ISEVRSDRDKFVIFLDVKHF SPEDLTVKVQDDFVEIHGKH NERQDDHGYISREFHRRYRL PSNVDQSALSCSLSADGMLT FCGPKIQTGLDATHAERAIP VSREEKPTSAPSS

Data sets:
Data typeCount
13C chemical shifts81
15N chemical shifts22
1H chemical shifts111

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1alphaA-crystallin (or HSPB1)1

Entities:

Entity 1, alphaA-crystallin (or HSPB1) 173 residues - Formula weight is not available

1   METASPVALTHRILEGLNHISPROTRPPHE
2   LYSARGTHRLEUGLYPROPHETYRPROSER
3   ARGLEUPHEASPGLNPHEPHEGLYGLUGLY
4   LEUPHEGLUTYRASPLEULEUPROPHELEU
5   SERSERTHRILESERPROTYRTYRARGGLN
6   SERLEUPHEARGTHRVALLEUASPSERGLY
7   ILESERGLUVALARGSERASPARGASPLYS
8   PHEVALILEPHELEUASPVALLYSHISPHE
9   SERPROGLUASPLEUTHRVALLYSVALGLN
10   ASPASPPHEVALGLUILEHISGLYLYSHIS
11   ASNGLUARGGLNASPASPHISGLYTYRILE
12   SERARGGLUPHEHISARGARGTYRARGLEU
13   PROSERASNVALASPGLNSERALALEUSER
14   CYSSERLEUSERALAASPGLYMETLEUTHR
15   PHECYSGLYPROLYSILEGLNTHRGLYLEU
16   ASPALATHRHISALAGLUARGALAILEPRO
17   VALSERARGGLUGLULYSPROTHRSERALA
18   PROSERSER

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