BMRB Entry 27120
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27120
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Title: Backbone chemical shifts of a circularized type III antifreeze protein from Macrozoarces americanus PubMed: 28691252
Deposition date: 2017-05-31 Original release date: 2017-07-06
Authors: Stevens, Corey; Langelaan, David; Allingham, John; Davies, Peter
Citation: Stevens, Corey; Semrau, Joanna; Chiriac, Dragos; Litschko, Morgan; Campbell, Robert; Langelaan, David; Smith, Steven; Davies, Peter; Allingham, John. "Peptide backbone circularization enhances antifreeze protein thermostability." Protein Sci. 26, 1932-1941 (2017).
Assembly members:
circAFP, polymer, 74 residues, Formula weight is not available
Natural source: Common Name: ocean pout Taxonomy ID: 8199 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Macrozoarces americanus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24
Entity Sequences (FASTA):
circAFP: CWEGAANQASVVANQLIPIN
TALTLVMMRSEVVTPVGIPA
EDIPRLVSMQVNRAVPLGTT
LMPDMVKGYAARGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 216 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | circAFP | 1 |
Entities:
Entity 1, circAFP 74 residues - Formula weight is not available
This is a circularized protein.
| 1 | CYS | TRP | GLU | GLY | ALA | ALA | ASN | GLN | ALA | SER | ||||
| 2 | VAL | VAL | ALA | ASN | GLN | LEU | ILE | PRO | ILE | ASN | ||||
| 3 | THR | ALA | LEU | THR | LEU | VAL | MET | MET | ARG | SER | ||||
| 4 | GLU | VAL | VAL | THR | PRO | VAL | GLY | ILE | PRO | ALA | ||||
| 5 | GLU | ASP | ILE | PRO | ARG | LEU | VAL | SER | MET | GLN | ||||
| 6 | VAL | ASN | ARG | ALA | VAL | PRO | LEU | GLY | THR | THR | ||||
| 7 | LEU | MET | PRO | ASP | MET | VAL | LYS | GLY | TYR | ALA | ||||
| 8 | ALA | ARG | GLY | LYS |
Samples:
sample_1: circAFP, [U-100% 13C; U-100% 15N], 3 mM; TRIS 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 70 mM; pH: 7.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNmr_Analysis v2.4, CCPN, Varian - chemical shift assignment, collection, data analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts