BMRB Entry 27125

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27125

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Title:
FUS low complexity domain with 12 phosphomimetic S/T to E mutations
Deposition date:
2017-06-05
Original release date:
2017-08-02
Authors:
Ryan, Veronica; Fawzi, Nicolas; Burke, Kathleen
Citation:

Citation: Monahan, Zachary; Ryan, Veronica; Janke, Abigail; Burke, Kathleen; Rhoads, Shannon; Zerze, Gul; O'Meally, Robert; Dignon, Gregory; Conicella, Alexander; Zheng, Wenwei; Best, Robert; Cole, Robert; Mittal, Jeetain; Shewmaker, Frank; Fawzi, Nicolas. "Phosphorylation of the FUS low-complexity domain disrupts phase separation, aggregation, and toxicity"  EMBO J. 36, 2951-2967 (2017).
PubMed: 28790177

Assembly members:

Assembly members:
FUS_12E_LC, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: MBP-FUS_LC_12E_pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FUS_12E_LC: GMASNDYEQQAEQSYGAYPE QPGQGYEQQSEQPYGQQSYS GYEQSTDTSGYGQSSYSSYG QEQNTGYGEQSTPQGYGSTG GYGSEQSEQSSYGQQSSYPG YGQQPAPSSTSGSYGSSEQS SSYGQPQSGSYEQQPSYGGQ QQSYGQQQSYNPPQGYGQQN QYNS

Data sets:
Data typeCount
13C chemical shifts423
15N chemical shifts151
1H chemical shifts151

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1FUS 12E LC1

Entities:

Entity 1, FUS 12E LC 164 residues - Formula weight is not available

Residue 1 is cleavage artifact, reside 2 is residue 1 in FUS

1   GLYMETALASERASNASPTYRGLUGLNGLN
2   ALAGLUGLNSERTYRGLYALATYRPROGLU
3   GLNPROGLYGLNGLYTYRGLUGLNGLNSER
4   GLUGLNPROTYRGLYGLNGLNSERTYRSER
5   GLYTYRGLUGLNSERTHRASPTHRSERGLY
6   TYRGLYGLNSERSERTYRSERSERTYRGLY
7   GLNGLUGLNASNTHRGLYTYRGLYGLUGLN
8   SERTHRPROGLNGLYTYRGLYSERTHRGLY
9   GLYTYRGLYSERGLUGLNSERGLUGLNSER
10   SERTYRGLYGLNGLNSERSERTYRPROGLY
11   TYRGLYGLNGLNPROALAPROSERSERTHR
12   SERGLYSERTYRGLYSERSERGLUGLNSER
13   SERSERTYRGLYGLNPROGLNSERGLYSER
14   TYRGLUGLNGLNPROSERTYRGLYGLYGLN
15   GLNGLNSERTYRGLYGLNGLNGLNSERTYR
16   ASNPROPROGLNGLYTYRGLYGLNGLNASN
17   GLNTYRASNSER

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