BMRB Entry 27130

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27130

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Title:
1H, 13C, and 15N chemical shift assignments of the FnIII-2 domain from Vibrio cholerae RbmA
Deposition date:
2017-06-09
Original release date:
2017-09-14
Authors:
Parsley, Nicole; Lee, Hsiau-Wei; Partch, Carrie
Citation:

Citation: Fong, Jiunn; Rogers, Andrew; Michael, Alicia; Parsley, Nicole; Cornell, William-Cole; Lin, Yu-Cheng; Singh, Praveen; Hartmann, Raimo; Drescher, Knut; Vinogradov, Evgeny; Dietrich, Lars; Partch, Carrie; Yildiz, Fitnat. "Structural dynamics of RbmA governs plasticity of Vibrio cholerae biofilms"  Elife 6, e26163-e26163 (2017).

Assembly members:

Assembly members:
RbmA_FnIII-2_domain, polymer, 117 residues, 12683.23 Da.

Natural source:

Natural source:   Common Name: Vibrio cholerae   Taxonomy ID: 666   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Vibrio cholerae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHisGST-TEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RbmA_FnIII-2_domain: GAMDPELNGLTIDIKNQFGI NSVESTGGFVPFTVDLNNGR EGEANVEFWMTAVGPDGLII PVNAREKWVIASGDTYSKVR GINFDKSYPAGEYTINAQVV DIVSGERVEQSMTVVKK

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts121
1H chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RbmA FnIII-2 domain1

Entities:

Entity 1, RbmA FnIII-2 domain 117 residues - 12683.23 Da.

Residues 155-159 represent a vector artifact (GAMDP) left after TEV cleavage

1   GLYALAMETASPPROGLULEUASNGLYLEU
2   THRILEASPILELYSASNGLNPHEGLYILE
3   ASNSERVALGLUSERTHRGLYGLYPHEVAL
4   PROPHETHRVALASPLEUASNASNGLYARG
5   GLUGLYGLUALAASNVALGLUPHETRPMET
6   THRALAVALGLYPROASPGLYLEUILEILE
7   PROVALASNALAARGGLULYSTRPVALILE
8   ALASERGLYASPTHRTYRSERLYSVALARG
9   GLYILEASNPHEASPLYSSERTYRPROALA
10   GLYGLUTYRTHRILEASNALAGLNVALVAL
11   ASPILEVALSERGLYGLUARGVALGLUGLN
12   SERMETTHRVALVALLYSLYS

Related Database Links:

GB APF52364.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks