BMRB Entry 27134

Assembly:

Entities:

Entity 1, MSP2 (172-221) 58 residues - 6300 Da.

First 50 resides are conserved C-terminal MSP2 and this construct has two extra residues (Leu, Glu) from the vector between the protein and His6-tag, giving a total of 58 residues.

Samples:

sample_4: C-terminal region of MSP2, [U-99% 13C; U-98% 15N], 1.25 mM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v; Dodecylphosphocholine, [U-98% 2H], 200 mM; DOGS-NTA 2 mM; Nickel sulphate 1.5 mM

sample_1: C-terminal domain of MSP2, [U-98% 15N], 100 uM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v

sample_2: C-terminal domain of MSP2, [U-98% 15N], 100 uM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v; DPC, [U-98% 2H], 100 mM; DOGS-NTA 1 mM; Nickel sulphate 0.2 mM

sample_3: C-terminal domain of MSP2, [U-99% 13C; U-98% 15N], 1.25 mM; Sodium acetate, [U-99% 2H], 20 mM; D2O, [U-100% 2H], 5 % v/v

sample_conditions_1: pH: 4.5; temperature: 298 K

Experiments:

Software:

TOPSPIN v2.3, Bruker Biospin - collection, processing

CcpNMR v2.4.2, CCPN - chemical shift assignment

NMR spectrometers:

• Bruker Avance lll 600 MHz