BMRB Entry 27145
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27145
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Title: Backbone 1H, 13C, 15N assignments for the chromoshadow domain of S. pombe Swi6 PubMed: 28942089
Deposition date: 2017-06-17 Original release date: 2017-09-29
Authors: Gross, John; Sanulli, Serena; Tibble, Ryan; Isaac, R Stefan; Narlikar, Geeta
Citation: Stefan Isaac, R.; Sanulli, Serena; Tibble, Ryan; Hornsby, Michael; Ravalin, Matthew; Craik, Charles; Gross, John; Narlikar, Geeta. "Biochemical basis for distinct roles of the heterochromatin proteins Swi6 and Chp2" J. Mol. Biol. ., 30449-30449 (2017).
Assembly members:
Swi6, polymer, 69 residues, Formula weight is not available
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30a
Entity Sequences (FASTA):
Swi6: GKQVENYDSWEDLVSSIDTI
ERKDDGTLEIYLTWKNGAIS
HHPSTITNKKCPQKMLQFYE
SHLTFRENE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 106 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 55 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | chromatin-associated portein swi6 | 1 |
Entities:
Entity 1, chromatin-associated portein swi6 69 residues - Formula weight is not available
| 1 | GLY | LYS | GLN | VAL | GLU | ASN | TYR | ASP | SER | TRP | ||||
| 2 | GLU | ASP | LEU | VAL | SER | SER | ILE | ASP | THR | ILE | ||||
| 3 | GLU | ARG | LYS | ASP | ASP | GLY | THR | LEU | GLU | ILE | ||||
| 4 | TYR | LEU | THR | TRP | LYS | ASN | GLY | ALA | ILE | SER | ||||
| 5 | HIS | HIS | PRO | SER | THR | ILE | THR | ASN | LYS | LYS | ||||
| 6 | CYS | PRO | GLN | LYS | MET | LEU | GLN | PHE | TYR | GLU | ||||
| 7 | SER | HIS | LEU | THR | PHE | ARG | GLU | ASN | GLU |
Samples:
Swi6_CSD: Swi6, [U-100% 13C; U-100% 15N], 250 uM; HEPES pH 7.8 20 mM; potassium chloride 150 mM; DTT 2 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | Swi6_CSD | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | Swi6_CSD | isotropic | sample_conditions_1 |
| 3D HNCA | Swi6_CSD | isotropic | sample_conditions_1 |
Software:
SPARKY v3.131, Bahrami, Markley, Assadi, and Eghbalnia, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, peak picking, processing
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker DRX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts