BMRB Entry 27163

Name: 3Jcgn &amp; 3Jcgc&#039; scalar couplings for hnRNP A1 RRM1
Published: 2022-06-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27163

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

3Jcgn & 3Jcgc' scalar couplings for hnRNP A1 RRM1

Deposition date:

2017-06-27

Original release date:

2022-06-23

Authors:

Diarra dit Konte, N.; Damberger, F.; Allain, F.

Citation:

Citation: Diarra dit Konte, N.; Krepl, M.; Damberger, F.; Ripin, N.; Duss, O.; Sponer, J.; Allain, F.. "Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination" Nat. Commun. 8, 654-654 (2017).
PubMed: 28935965

Assembly members:

Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MASKSESPKEPEQLRKLFIG GLSFETTDESLRSHFEQWGT LTDCVVMRDPNTKRSRGFGF VTYATVEEVDAAMNARPHKV DGRVVEPKRAVSREDSQR

Data sets:

coupling_constants
- coupling_constants_1

Data type	Count
coupling constants	15

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 118 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ALA

SER

LYS

SER

GLU

SER

PRO

LYS

GLU

4

PRO

GLU

GLN

LEU

ARG

LYS

LEU

PHE

ILE

GLY

5

GLY

LEU

SER

PHE

GLU

THR

ASP

GLU

SER

6

LEU

ARG

SER

HIS

PHE

GLU

GLN

TRP

GLY

THR

7

LEU

THR

ASP

CYS

VAL

MET

ARG

ASP

PRO

8

ASN

THR

LYS

ARG

SER

ARG

GLY

PHE

GLY

PHE

9

VAL

THR

TYR

ALA

THR

VAL

GLU

VAL

ASP

10

ALA

MET

ASN

ALA

ARG

PRO

HIS

LYS

VAL

11

ASP

GLY

ARG

VAL

GLU

PRO

LYS

ARG

ALA

12

VAL

SER

ARG

GLU

ASP

SER

GLN

ARG

Samples:

sample_1: entity_1, [U-99% 13C; U-99% 15N], 2 mM; H2O 90 % v/v; D2O 10 % v/v; sodium phosphate 10 mM; beta-mercaptoethanol 10 mM; DEPC 0.1 % v/v

sample_conditions_1: ionic strength: 130 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
spin-echo difference constant time 15N-HSQC	sample_1	isotropic	sample_conditions_1
spin-echo difference constant time HN(CO)CG	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker AvanceIII 500 MHz