BMRB Entry 27164
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27164
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Title: 3Jcgn & 3Jcgc' scalar couplings for hnRNP A1 RRM1 PubMed: 28935965
Deposition date: 2017-06-27 Original release date: 2022-06-23
Authors: Diarra dit Konte, N.; Damberger, F.; Allain, F.
Citation: Diarra dit Konte, N.; Krepl, M.; Damberger, F.; Ripin, N.; Duss, O.; Sponer, J.; Allain, F.. "Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination" Nat. Commun. 8, 654-654 (2017).
Assembly members:
entity_1, polymer, 118 residues, Formula weight is not available
entity_2, polymer, 7 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a+
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH
MASKSESPKEPEQLRKLFIG
GLSFETTDESLRSHFEQWGT
LTDCVVMRDPNTKRSRGFGF
VTYATVEEVDAAMNARPHKV
DGRVVEPKRAVSREDSQR
entity_2: UUAGGCU
- coupling_constants
| Data type | Count |
| coupling constants | 14 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hnRNP A1 RRM1 | 1 |
| 2 | UUAGGCU | 2 |
Entities:
Entity 1, hnRNP A1 RRM1 118 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ALA | SER | LYS | SER | GLU | SER | PRO | LYS | GLU | ||||
| 4 | PRO | GLU | GLN | LEU | ARG | LYS | LEU | PHE | ILE | GLY | ||||
| 5 | GLY | LEU | SER | PHE | GLU | THR | THR | ASP | GLU | SER | ||||
| 6 | LEU | ARG | SER | HIS | PHE | GLU | GLN | TRP | GLY | THR | ||||
| 7 | LEU | THR | ASP | CYS | VAL | VAL | MET | ARG | ASP | PRO | ||||
| 8 | ASN | THR | LYS | ARG | SER | ARG | GLY | PHE | GLY | PHE | ||||
| 9 | VAL | THR | TYR | ALA | THR | VAL | GLU | GLU | VAL | ASP | ||||
| 10 | ALA | ALA | MET | ASN | ALA | ARG | PRO | HIS | LYS | VAL | ||||
| 11 | ASP | GLY | ARG | VAL | VAL | GLU | PRO | LYS | ARG | ALA | ||||
| 12 | VAL | SER | ARG | GLU | ASP | SER | GLN | ARG |
Entity 2, UUAGGCU 7 residues - Formula weight is not available
| 1 | U | U | A | G | G | C | U |
Samples:
sample_1: entity_1, [U-99% 13C; U-99% 15N], 1.2 mM; H2O 90 % v/v; D2O 10 % v/v; sodium phosphate 10 mM; beta-mercaptoethanol 10 mM; DEPC 0.1 % v/v; entity_2 1.2 mM
sample_conditions_1: ionic strength: 130 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| spin-echo difference constant time 15N-HSQC | sample_1 | isotropic | sample_conditions_1 |
| spin-echo difference constant time HN(CO)CG | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker AvanceIII 500 MHz