BMRB Entry 27171
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27171
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Title: FXN_AIN_ISCU PubMed: 29576242
Deposition date: 2017-07-10 Original release date: 2017-07-12
Authors: Cai, Kai; Markley, John
Citation: Cai, Kai; Frederick, Ronnie; Tonelli, Marco; Markley, John. "Interactions of iron-bound frataxin with ISCU and ferredoxin on the cysteine desulfurase complex leading to Fe-S cluster assembly." J. Inorg. Biochem. 183, 107-116 (2018).
Assembly members:
Frataxin, polymer, 130 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO
Entity Sequences (FASTA):
Frataxin: SGTLGHPGSLDETTYERLAE
ETLDSLAEFFEDLADKPYTF
EDYDVSFGSGVLTVKLGGDL
GTYVINKQTPNKQIWLSSPS
SGPKRYDWTGKNWVFSHDGV
SLHELLAAELTKALKTKLDL
SWLAYSGKDA
| Data type | Count |
| . | . |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Frataxin Mature Version | 1 |
Entities:
Entity 1, Frataxin Mature Version 130 residues - Formula weight is not available
| 1 | SER | GLY | THR | LEU | GLY | HIS | PRO | GLY | SER | LEU | |
| 2 | ASP | GLU | THR | THR | TYR | GLU | ARG | LEU | ALA | GLU | |
| 3 | GLU | THR | LEU | ASP | SER | LEU | ALA | GLU | PHE | PHE | |
| 4 | GLU | ASP | LEU | ALA | ASP | LYS | PRO | TYR | THR | PHE | |
| 5 | GLU | ASP | TYR | ASP | VAL | SER | PHE | GLY | SER | GLY | |
| 6 | VAL | LEU | THR | VAL | LYS | LEU | GLY | GLY | ASP | LEU | |
| 7 | GLY | THR | TYR | VAL | ILE | ASN | LYS | GLN | THR | PRO | |
| 8 | ASN | LYS | GLN | ILE | TRP | LEU | SER | SER | PRO | SER | |
| 9 | SER | GLY | PRO | LYS | ARG | TYR | ASP | TRP | THR | GLY | |
| 10 | LYS | ASN | TRP | VAL | PHE | SER | HIS | ASP | GLY | VAL | |
| 11 | SER | LEU | HIS | GLU | LEU | LEU | ALA | ALA | GLU | LEU | |
| 12 | THR | LYS | ALA | LEU | LYS | THR | LYS | LEU | ASP | LEU | |
| 13 | SER | TRP | LEU | ALA | TYR | SER | GLY | LYS | ASP | ALA |
Samples:
sample_1: Frataxin, [U-100% 15N], 0.3 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 750 MHz