BMRB Entry 27179
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27179
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Hepatitis B Virus X protein (HBx)
Deposition date: 2017-07-14 Original release date: 2019-03-15
Authors: Delgado, Leonildo; Grzesiek, Stephan
Citation: Delgado, Leonildo; Grzesiek, Stephan; Delgado, Leonildo; Grzesiek, Stephan. "1H, 15N, 13C assignments and 15N relaxation data of the intrinsically disordered hepatitis B virus X protein (HBx) in a detergent mixture" The BMRB entry is the only known published source for the data..
Assembly members:
Hepatitis_B_virus_X_protein_(HBx), polymer, 177 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pROX180-9CS N-ter
Entity Sequences (FASTA):
Hepatitis_B_virus_X_protein_(HBx): MGSSHHHHHHSSGRENLYFQ
GSGSAARLSSQLDPARDVLS
LRPVGAESRGRPFSGSLGTL
SSPSPSAVPTDHGAHLSLRG
LPVSAFSSAGPSALRFTSAR
RMETTVNAHQILPKVLHKRT
LGLSAMSTTDLEAYFKDSLF
KDWEELGEEIRLKVFVLGGS
RHKLVSAPAPSNFFTSA
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 450 |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 629 |
| T1 relaxation values | 219 |
| T2 relaxation values | 219 |
| heteronuclear NOE values | 224 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Hepatitis B virus X protein (HBx) | 1 |
Entities:
Entity 1, Hepatitis B virus X protein (HBx) 177 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | ARG | GLU | ASN | LEU | TYR | PHE | GLN | ||||
| 3 | GLY | SER | GLY | SER | ALA | ALA | ARG | LEU | SER | SER | ||||
| 4 | GLN | LEU | ASP | PRO | ALA | ARG | ASP | VAL | LEU | SER | ||||
| 5 | LEU | ARG | PRO | VAL | GLY | ALA | GLU | SER | ARG | GLY | ||||
| 6 | ARG | PRO | PHE | SER | GLY | SER | LEU | GLY | THR | LEU | ||||
| 7 | SER | SER | PRO | SER | PRO | SER | ALA | VAL | PRO | THR | ||||
| 8 | ASP | HIS | GLY | ALA | HIS | LEU | SER | LEU | ARG | GLY | ||||
| 9 | LEU | PRO | VAL | SER | ALA | PHE | SER | SER | ALA | GLY | ||||
| 10 | PRO | SER | ALA | LEU | ARG | PHE | THR | SER | ALA | ARG | ||||
| 11 | ARG | MET | GLU | THR | THR | VAL | ASN | ALA | HIS | GLN | ||||
| 12 | ILE | LEU | PRO | LYS | VAL | LEU | HIS | LYS | ARG | THR | ||||
| 13 | LEU | GLY | LEU | SER | ALA | MET | SER | THR | THR | ASP | ||||
| 14 | LEU | GLU | ALA | TYR | PHE | LYS | ASP | SER | LEU | PHE | ||||
| 15 | LYS | ASP | TRP | GLU | GLU | LEU | GLY | GLU | GLU | ILE | ||||
| 16 | ARG | LEU | LYS | VAL | PHE | VAL | LEU | GLY | GLY | SER | ||||
| 17 | ARG | HIS | LYS | LEU | VAL | SER | ALA | PRO | ALA | PRO | ||||
| 18 | SER | ASN | PHE | PHE | THR | SER | ALA |
Samples:
sample_1: Hepatitis B virus X protein (HBx), [U-100% 13C; U-100% 15N], 0.7 mM; sodium acetate 20 mM; EDTA 2 mM; DHPC 100 mM; Fos-Choline-12 2.8 mM; sodium chloride 0 mM
sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 4D (H)NNH) | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, peak picking
PIPP, Garrett - peak picking
NMR spectrometers:
- Bruker Avance 900 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts