BMRB Entry 27189

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27189

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
N,H,Ca,Cb chemical shifts and methionine sidechain chemical shifts of the isolated P1 domain of CheA from Escherichia Coli
Deposition date:
2017-07-21
Original release date:
2017-12-14
Authors:
Minato, Yuichi; Ueda, Takumi; Machiyama, Asako; Iwai, Hideo; Shimada, Ichio
Citation:

Citation: Minato, Yuichi; Ueda, Takumi; Machiyama, Asako; Iwai, Hideo; Shimada, Ichio. "Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR"  Sci. Rep. 7, 16462-16462 (2017).
PubMed: 29184123

Assembly members:

Assembly members:
P1_domain_of_CheA, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET43a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
P1_domain_of_CheA: MDISDFYQTFFDEADELLAD MEQHLLVLQPEAPDAEQLNA IFRAAHSIKGGAGTFGFSVL QETTHLMENLLDEARRGEMQ LNTDIINLFLETKDIMQEQL DAYKQSQEPDAASFDYICQA LRQLALEAKGET

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts128
1H chemical shifts168

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P1 domain of CheA1

Entities:

Entity 1, P1 domain of CheA 132 residues - Formula weight is not available

1   METASPILESERASPPHETYRGLNTHRPHE
2   PHEASPGLUALAASPGLULEULEUALAASP
3   METGLUGLNHISLEULEUVALLEUGLNPRO
4   GLUALAPROASPALAGLUGLNLEUASNALA
5   ILEPHEARGALAALAHISSERILELYSGLY
6   GLYALAGLYTHRPHEGLYPHESERVALLEU
7   GLNGLUTHRTHRHISLEUMETGLUASNLEU
8   LEUASPGLUALAARGARGGLYGLUMETGLN
9   LEUASNTHRASPILEILEASNLEUPHELEU
10   GLUTHRLYSASPILEMETGLNGLUGLNLEU
11   ASPALATYRLYSGLNSERGLNGLUPROASP
12   ALAALASERPHEASPTYRILECYSGLNALA
13   LEUARGGLNLEUALALEUGLUALALYSGLY
14   GLUTHR

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks