BMRB Entry 27189

Name: N,H,Ca,Cb chemical shifts and methionine sidechain chemical shifts of the isolated P1 domain of CheA from Escherichia Coli
Published: 2017-12-14

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27189

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: N,H,Ca,Cb chemical shifts and methionine sidechain chemical shifts of the isolated P1 domain of CheA from Escherichia Coli PubMed: 29184123

Deposition date: 2017-07-21 Original release date: 2017-12-14

Authors: Minato, Yuichi; Ueda, Takumi; Machiyama, Asako; Iwai, Hideo; Shimada, Ichio

Citation: Minato, Yuichi; Ueda, Takumi; Machiyama, Asako; Iwai, Hideo; Shimada, Ichio. "Dynamic domain arrangement of CheA-CheY complex regulates bacterial thermotaxis, as revealed by NMR" Sci. Rep. 7, 16462-16462 (2017).

Assembly members:
P1_domain_of_CheA, polymer, 132 residues, Formula weight is not available

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET43a

Entity Sequences (FASTA):
P1_domain_of_CheA: MDISDFYQTFFDEADELLAD MEQHLLVLQPEAPDAEQLNA IFRAAHSIKGGAGTFGFSVL QETTHLMENLLDEARRGEMQ LNTDIINLFLETKDIMQEQL DAYKQSQEPDAASFDYICQA LRQLALEAKGET

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	254
15N chemical shifts	128
1H chemical shifts	168

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	P1 domain of CheA	1

Entities:

Entity 1, P1 domain of CheA 132 residues - Formula weight is not available

1

MET

ASP

ILE

SER

ASP

PHE

TYR

GLN

THR

PHE

2

PHE

ASP

GLU

ALA

ASP

GLU

LEU

ALA

ASP

3

MET

GLU

GLN

HIS

LEU

VAL

LEU

GLN

PRO

4

GLU

ALA

PRO

ASP

ALA

GLU

GLN

LEU

ASN

ALA

5

ILE

PHE

ARG

ALA

HIS

SER

ILE

LYS

GLY

6

GLY

ALA

GLY

THR

PHE

GLY

PHE

SER

VAL

LEU

7

GLN

GLU

THR

HIS

LEU

MET

GLU

ASN

LEU

8

LEU

ASP

GLU

ALA

ARG

GLY

GLU

MET

GLN

9

LEU

ASN

THR

ASP

ILE

ASN

LEU

PHE

LEU

10

GLU

THR

LYS

ASP

ILE

MET

GLN

GLU

GLN

LEU

11

ASP

ALA

TYR

LYS

GLN

SER

GLN

GLU

PRO

ASP

12

ALA

SER

PHE

ASP

TYR

ILE

CYS

GLN

ALA

13

LEU

ARG

GLN

LEU

ALA

LEU

GLU

ALA

LYS

GLY

14

GLU

THR

Samples:

sample_1: P1 domain of CheA, [U-13C; U-15N], 0.78 mM; sodium phosphate 20 mM; DTT 2 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 7.1; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts