BMRB Entry 27209

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27209

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Title:
1HN, 13C, and 15N Resonance Assignments of the human DNA Ligase 3 DNA-Binding Domain (residues 257-477)
Deposition date:
2017-08-08
Original release date:
2019-07-10
Authors:
Roth, Braden; Varney, Kristen; Weber, David; Tomkinson, Alan
Citation:

Citation: Roth, Braden; Varney, Kristen; Yang, Hui; Weber, David; Tomkinson, Alan. "1HN, 13C, and 15N backbone resonance assignments of the human DNA ligase 3 DNA-binding domain (residues 257-477)"  Biomol. NMR Assignments 13, 305-308 (2019).
PubMed: 31093909

Assembly members:

Assembly members:
LIG3DBD, polymer, 222 residues, 25195.8182 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHGK-LIG3DBD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIG3DBD: SRHKDCLLREFRKLCAMVAD NPSYNTKTQIIQDFLRKGSA GDGFHGDVYLTVKLLLPGVI KTVYNLNDKQIVKLFSRIFN CNPDDMARDLEQGDVSETIR VFFEQSKSFPPAAKSLLTIQ EVDEFLLRLSKLTKEDEQQQ ALQDIASRCTANDLKCIIRL IKHDLKMNSGAKHVLDALDP NAYEAFKASRNLQDVVERVL HNAQEVEKEPGQRRALSVQA SL

Data sets:
Data typeCount
13C chemical shifts589
15N chemical shifts186
1H chemical shifts186

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LIG3DBD1

Entities:

Entity 1, LIG3DBD 222 residues - 25195.8182 Da.

residue 1 represents a non-native amino acid resulting from TEV cleavage of the protein from a fusion partner

1   SERARGHISLYSASPCYSLEULEUARGGLU
2   PHEARGLYSLEUCYSALAMETVALALAASP
3   ASNPROSERTYRASNTHRLYSTHRGLNILE
4   ILEGLNASPPHELEUARGLYSGLYSERALA
5   GLYASPGLYPHEHISGLYASPVALTYRLEU
6   THRVALLYSLEULEULEUPROGLYVALILE
7   LYSTHRVALTYRASNLEUASNASPLYSGLN
8   ILEVALLYSLEUPHESERARGILEPHEASN
9   CYSASNPROASPASPMETALAARGASPLEU
10   GLUGLNGLYASPVALSERGLUTHRILEARG
11   VALPHEPHEGLUGLNSERLYSSERPHEPRO
12   PROALAALALYSSERLEULEUTHRILEGLN
13   GLUVALASPGLUPHELEULEUARGLEUSER
14   LYSLEUTHRLYSGLUASPGLUGLNGLNGLN
15   ALALEUGLNASPILEALASERARGCYSTHR
16   ALAASNASPLEULYSCYSILEILEARGLEU
17   ILELYSHISASPLEULYSMETASNSERGLY
18   ALALYSHISVALLEUASPALALEUASPPRO
19   ASNALATYRGLUALAPHELYSALASERARG
20   ASNLEUGLNASPVALVALGLUARGVALLEU
21   HISASNALAGLNGLUVALGLULYSGLUPRO
22   GLYGLNARGARGALALEUSERVALGLNALA
23   SERLEU

Related Database Links:

UniProt P49916
AlphaFold Q6NVK3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks