BMRB Entry 27210
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27210
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Title: PhoBN F20D assignment PubMed: 29299752
Deposition date: 2017-08-08 Original release date: 2018-02-02
Authors: Kou, Xinhui
Citation: Kou, Xinhui; Liu, Xinghong; Liu, Yixiang; Li, Conggang; Liu, Maili; Jiang, Ling. "Backbone resonance assignment of the Escherichia coli protein mutation PhoBNF20D" Biomol. NMR Assign. 12, 133-137 (2018).
Assembly members:
PhoBNF20D, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET28a
Entity Sequences (FASTA):
PhoBNF20D: MARRILVVEDEAPIREMVCD
VLEQNGFQPVEAEDYDSAVN
QLNEPWPDLILLDWMLPGGS
GIQFIKHLKRESMTRDIPVV
MLTARGEEEDRVRGLETGAD
DYITKPFSPKELVARIKAVM
RRISQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 218 |
| 15N chemical shifts | 109 |
| 1H chemical shifts | 205 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PhoBNF20D | 1 |
Entities:
Entity 1, PhoBNF20D 125 residues - Formula weight is not available
| 1 | MET | ALA | ARG | ARG | ILE | LEU | VAL | VAL | GLU | ASP | ||||
| 2 | GLU | ALA | PRO | ILE | ARG | GLU | MET | VAL | CYS | ASP | ||||
| 3 | VAL | LEU | GLU | GLN | ASN | GLY | PHE | GLN | PRO | VAL | ||||
| 4 | GLU | ALA | GLU | ASP | TYR | ASP | SER | ALA | VAL | ASN | ||||
| 5 | GLN | LEU | ASN | GLU | PRO | TRP | PRO | ASP | LEU | ILE | ||||
| 6 | LEU | LEU | ASP | TRP | MET | LEU | PRO | GLY | GLY | SER | ||||
| 7 | GLY | ILE | GLN | PHE | ILE | LYS | HIS | LEU | LYS | ARG | ||||
| 8 | GLU | SER | MET | THR | ARG | ASP | ILE | PRO | VAL | VAL | ||||
| 9 | MET | LEU | THR | ALA | ARG | GLY | GLU | GLU | GLU | ASP | ||||
| 10 | ARG | VAL | ARG | GLY | LEU | GLU | THR | GLY | ALA | ASP | ||||
| 11 | ASP | TYR | ILE | THR | LYS | PRO | PHE | SER | PRO | LYS | ||||
| 12 | GLU | LEU | VAL | ALA | ARG | ILE | LYS | ALA | VAL | MET | ||||
| 13 | ARG | ARG | ILE | SER | GLN |
Samples:
sample_1: PhoBNF20D, [U-98% 15N], 0.6 mM; PhoBNF20D, [U-95% 13C], 0.6 mM; soduim chloride 100 mM; DTT 10 mM; HEPES 20 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Uniform NMR System 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts