BMRB Entry 27215

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27215

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Title:
Chemical shift assignments of free prothymosin alpha
Deposition date:
2017-08-14
Original release date:
2018-02-12
Authors:
Bugge, Katrine; Fernandes, Catarina; Kragelund, Birthe; Borgia, Alessandro; Borgia, Madeleine; Heidarsson, Petur; Schuler, Benjamin
Citation:

Citation: Borgia, Alessandro; Borgia, Madeleine; Bugge, Katrine; Kissling, Vera; Heidarsson, Petur; Fernandes, Catarina; Sottini, Andrea; Soranno, Andrea; Buholzer, Karin; Nettels, Daniel; Kragelund, Birthe; Best, Robert; Schuler, Benjamin. "Extreme disorder in an ultrahigh-affinity protein complex."  Nature 555, 61-66 (2018).
PubMed: 29466338

Assembly members:

Assembly members:
Prothymosin_alpha, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET47b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Prothymosin_alpha: GPMSDAAVDTSSEITTKDLK EKKEVVEEAENGRDAPANGN ANEENGEQEADNEVDEEEEE GGEEEEEEEEGDGEEEDGDE DEEAESATGKRAAEDDEDDD VDTKKQKTDEDD

Data sets:
Data typeCount
13C chemical shifts279
15N chemical shifts105
1H chemical shifts169

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1free prothymosin alpha1

Entities:

Entity 1, free prothymosin alpha 112 residues - Formula weight is not available

The N-terminal "GP" is a tag cleavage leftover which is not counted in the residue numbering (1Met).

1   GLYPROMETSERASPALAALAVALASPTHR
2   SERSERGLUILETHRTHRLYSASPLEULYS
3   GLULYSLYSGLUVALVALGLUGLUALAGLU
4   ASNGLYARGASPALAPROALAASNGLYASN
5   ALAASNGLUGLUASNGLYGLUGLNGLUALA
6   ASPASNGLUVALASPGLUGLUGLUGLUGLU
7   GLYGLYGLUGLUGLUGLUGLUGLUGLUGLU
8   GLYASPGLYGLUGLUGLUASPGLYASPGLU
9   ASPGLUGLUALAGLUSERALATHRGLYLYS
10   ARGALAALAGLUASPASPGLUASPASPASP
11   VALASPTHRLYSLYSGLNLYSTHRASPGLU
12   ASPASP

Related Database Links:

UNP P06454
AlphaFold Q15592

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks