BMRB Entry 27227

Name: Backbone 1H and 15N Chemical Shift Assignments for mSin3B
Published: 2018-10-09

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PDB ID: 5y95
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27227
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H and 15N Chemical Shift Assignments for mSin3B

Deposition date:

2017-08-22

Original release date:

2018-10-09

Authors:

Kurita, Junichi; Hirao, Yuuka; Nishimura, Yoshifumi

Citation:

Citation: Ueda, Hiroshi; Kurita, Jun-Ichi; Neyama, Hiroyuki; Hirao, Yuuka; Kouji, Hiroyuki; Mishina, Tadashi; Kasai, Masaji; Nakano, Hirofumi; Yoshimori, Atsushi; Nishimura, Yoshifumi. "A mimetic of the mSin3-binding helix of NRSF/REST ameliorates abnormal pain behavior in chronic pain models" Bioorg. Med. Chem. Lett. 27, 4705-4709 (2017).
PubMed: 28927787

Assembly members:

Assembly members:
mSin3B, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mSin3B: GSHMEKLPVHVEDALTYLDQ VKIRFGSDPATYNGFLEIMK EFKSQSIDTPGVIRRVSQLF HEHPDLIVGFNAFLPLGYRI DIPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	46
1H chemical shifts	47

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mSin3B PAH domain	1

Entities:

Entity 1, mSin3B PAH domain 84 residues - Formula weight is not available

1

GLY

SER

HIS

MET

GLU

LYS

LEU

PRO

VAL

HIS

2

VAL

GLU

ASP

ALA

LEU

THR

TYR

LEU

ASP

GLN

3

VAL

LYS

ILE

ARG

PHE

GLY

SER

ASP

PRO

ALA

4

THR

TYR

ASN

GLY

PHE

LEU

GLU

ILE

MET

LYS

5

GLU

PHE

LYS

SER

GLN

SER

ILE

ASP

THR

PRO

6

GLY

VAL

ILE

ARG

VAL

SER

GLN

LEU

PHE

7

HIS

GLU

HIS

PRO

ASP

LEU

ILE

VAL

GLY

PHE

8

ASN

ALA

PHE

LEU

PRO

LEU

GLY

TYR

ARG

ILE

9

ASP

ILE

PRO

LYS

Samples:

sample_1: mSin3B, [U-98% 15N], 100 uM

sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRviewJ, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker AVANCEIII 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks