BMRB Entry 27230

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27230

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Title:
Elastin-like Protein 40 (ELP40)
Deposition date:
2017-08-22
Original release date:
2018-04-18
Authors:
Zhang, Yue; Fitzkee, Nicholas
Citation:

Citation: Zhang, Yue; Zai-Rose, Valeria; Price, Cody; Ezzell, Nicholas; Bidwell, Gene; Correia, John; Fitzkee, Nicholas. "Modeling the Early Stages of Phase Separation in Disordered Elastin-like Proteins."  Biophys. J. 114, 1563-1578 (2018).
PubMed: 29642027

Assembly members:

Assembly members:
Elastin-like_Protein_40_(ELP40), polymer, 207 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: purified from the natural source   Host organism: Escherichia coli   Vector: pET-25b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Elastin-like_Protein_40_(ELP40): VPGKGVPGVGVPGVGVPGVG VPGVGVPGVGVPGVGVPGVG VPGTGVPGVGVPGVGVPGVG VPGVGVPGVGVPGVGVPGVG VPGAGVPGVGVPGVGVPGVG VPGVGVPGVGVPGVGVPGVG VPGIGVPGVGVPGVGVPGVG VPGVGVPGVGVPGVGVPGVG VPGSGVPGVGVPGVGVPGVG VPGVGVPGVGVPGVGVPGVG VPGLGWP

Data typeCount
13C chemical shifts1236
15N chemical shifts414
1H chemical shifts1420

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ELP40, trans conformer1
2ELP40, cis conformer1

Entities:

Entity 1, ELP40, trans conformer 207 residues - Formula weight is not available

This is a elastin-like protein, which has 41 repeats.

1   VALPROGLYLYSGLYVALPROGLYVALGLY
2   VALPROGLYVALGLYVALPROGLYVALGLY
3   VALPROGLYVALGLYVALPROGLYVALGLY
4   VALPROGLYVALGLYVALPROGLYVALGLY
5   VALPROGLYTHRGLYVALPROGLYVALGLY
6   VALPROGLYVALGLYVALPROGLYVALGLY
7   VALPROGLYVALGLYVALPROGLYVALGLY
8   VALPROGLYVALGLYVALPROGLYVALGLY
9   VALPROGLYALAGLYVALPROGLYVALGLY
10   VALPROGLYVALGLYVALPROGLYVALGLY
11   VALPROGLYVALGLYVALPROGLYVALGLY
12   VALPROGLYVALGLYVALPROGLYVALGLY
13   VALPROGLYILEGLYVALPROGLYVALGLY
14   VALPROGLYVALGLYVALPROGLYVALGLY
15   VALPROGLYVALGLYVALPROGLYVALGLY
16   VALPROGLYVALGLYVALPROGLYVALGLY
17   VALPROGLYSERGLYVALPROGLYVALGLY
18   VALPROGLYVALGLYVALPROGLYVALGLY
19   VALPROGLYVALGLYVALPROGLYVALGLY
20   VALPROGLYVALGLYVALPROGLYVALGLY
21   VALPROGLYLEUGLYTRPPRO

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