BMRB Entry 27235

Name: Solution NMR assignment of the C-terminal domain of chTOG
Published: 2018-03-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27235

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Solution NMR assignment of the C-terminal domain of chTOG PubMed: 29582386

Deposition date: 2017-08-25 Original release date: 2018-03-29

Authors: Pfuhl, Mark

Citation: Rostkova, Elena; Burgess, Selena; Bayliss, Richard; Pfuhl, Mark. "Solution NMR assignment of the C-terminal domain of human chTOG" Biomol. NMR Assign. 12, 221-224 (2018).

Assembly members:
chTOG, polymer, 141 residues, 15.9 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET6a

Entity Sequences (FASTA):
chTOG: ASRIDEKSSKAKVNDFLAEI FKKIGSKENTKEGLAELYEY KKKYSDADIEPFLKNSSQFF QSYVERGLRVIEMEREGKGR ISTSTGISPQMEVTCVPTPT STVSSIGNTNGEEVGPSVYL ERLKILRQRCGLDNTKQDDR P

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	617
15N chemical shifts	142
1H chemical shifts	979

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminal domain of chTOG	1

Entities:

Entity 1, C-terminal domain of chTOG 141 residues - 15.9 Da.

the first residue A1817 is a cloning atifact

1

ALA

SER

ARG

ILE

ASP

GLU

LYS

SER

LYS

2

ALA

LYS

VAL

ASN

ASP

PHE

LEU

ALA

GLU

ILE

3

PHE

LYS

ILE

GLY

SER

LYS

GLU

ASN

THR

4

LYS

GLU

GLY

LEU

ALA

GLU

LEU

TYR

GLU

TYR

5

LYS

TYR

SER

ASP

ALA

ASP

ILE

GLU

6

PRO

PHE

LEU

LYS

ASN

SER

GLN

PHE

7

GLN

SER

TYR

VAL

GLU

ARG

GLY

LEU

ARG

VAL

8

ILE

GLU

MET

GLU

ARG

GLU

GLY

LYS

GLY

ARG

9

ILE

SER

THR

SER

THR

GLY

ILE

SER

PRO

GLN

10

MET

GLU

VAL

THR

CYS

VAL

PRO

THR

PRO

THR

11

SER

THR

VAL

SER

ILE

GLY

ASN

THR

ASN

12

GLY

GLU

VAL

GLY

PRO

SER

VAL

TYR

LEU

13

GLU

ARG

LEU

LYS

ILE

LEU

ARG

GLN

ARG

CYS

14

GLY

LEU

ASP

ASN

THR

LYS

GLN

ASP

ARG

15

PRO

Samples:

CTD_15N: chTOG, [U-98% 15N], 200 ± 10 uM; HEPES 20 ± 0.5 mM; sodium chloride 50 ± 1 mM; DTT 2 ± 0.1 mM

CTD_15N_13C: chTOG, [U-95% 13C; U-95% 15N], 200 ± 10 uM; HEPES 20 ± 0.5 mM; sodium chloride 50 ± 1 mM; DTT 2 ± 0.1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.10; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	CTD_15N	isotropic	sample_conditions_1
3D 1H-15N NOESY	CTD_15N	isotropic	sample_conditions_1
3D HNCACB	CTD_15N_13C	isotropic	sample_conditions_1
3D HNCO	CTD_15N_13C	isotropic	sample_conditions_1
3D CBCA(CO)NH	CTD_15N_13C	isotropic	sample_conditions_1
3D HBHA(CO)NH	CTD_15N_13C	isotropic	sample_conditions_1
3D H(CCO)NH	CTD_15N_13C	isotropic	sample_conditions_1
3D (H)C(CO)NH	CTD_15N_13C	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	CTD_15N_13C	isotropic	sample_conditions_1
2D 1H-13C TROSY aromatic	CTD_15N_13C	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	CTD_15N_13C	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	CTD_15N_13C	isotropic	sample_conditions_1

Software:

TOPSPIN v3.5, Bruker Biospin - collection, processing

CCPN_Analysis v2.4, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 800 MHz
Bruker Avance 950 MHz

SP	Q14008
AlphaFold	Q6NSH4

Biological Magnetic Resonance Data Bank