BMRB Entry 27237

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for mouse galectin-2
Published: 2018-02-08

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27237

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for mouse galectin-2 PubMed: 29392834

Deposition date: 2017-08-29 Original release date: 2018-02-08

Authors: Sakakura, Masayoshi; Tamura, Mayumi; Takeuchi, Tomoharu; Hatanaka, Tomomi; Arata, Yoichiro; Takahashi, Hideo

Citation: Sakakura, Masayoshi; Tamura, Mayumi; Fujii, Norihiko; Takeuchi, Tomoharu; Hatanaka, Tomomi; Kishimoto, Seishi; Arata, Yoichiro; Takahashi, Hideo. "Structural mechanisms for the S-nitrosylation-derived protection of mouse galectin-2 from oxidation-induced inactivation revealed by NMR" FEBS J. 285, 1129-1145 (2018).

Assembly members:
mGal-2, polymer, 130 residues, Formula weight is not available

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):
mGal-2: MSEKFEVKDLNMKPGMSLKI KGKIHNDVDRFLINLGQGKE TLNLHFNPRFDESTIVCNTS EGGRWGQEQRENHMCFSPGS EVKITITFQDKDFKVTLPDG HQLTFPNRLGHNQLHYLSMG GLQISSFKLE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	244
15N chemical shifts	121
1H chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protomer, mGal-2 chain 1	1
2	protomer, mGal-2 chain 2	1

Entities:

Entity 1, protomer, mGal-2 chain 1 130 residues - Formula weight is not available

1	MET	SER	GLU	LYS	PHE	GLU	VAL	LYS	ASP	LEU
2	ASN	MET	LYS	PRO	GLY	MET	SER	LEU	LYS	ILE
3	LYS	GLY	LYS	ILE	HIS	ASN	ASP	VAL	ASP	ARG
4	PHE	LEU	ILE	ASN	LEU	GLY	GLN	GLY	LYS	GLU
5	THR	LEU	ASN	LEU	HIS	PHE	ASN	PRO	ARG	PHE
6	ASP	GLU	SER	THR	ILE	VAL	CYS	ASN	THR	SER
7	GLU	GLY	GLY	ARG	TRP	GLY	GLN	GLU	GLN	ARG
8	GLU	ASN	HIS	MET	CYS	PHE	SER	PRO	GLY	SER
9	GLU	VAL	LYS	ILE	THR	ILE	THR	PHE	GLN	ASP
10	LYS	ASP	PHE	LYS	VAL	THR	LEU	PRO	ASP	GLY
11	HIS	GLN	LEU	THR	PHE	PRO	ASN	ARG	LEU	GLY
12	HIS	ASN	GLN	LEU	HIS	TYR	LEU	SER	MET	GLY
13	GLY	LEU	GLN	ILE	SER	SER	PHE	LYS	LEU	GLU

Samples:

sample_1: mGal-2, [U-99% 13C; U-98% 15N], 0.9 mM; MES 10 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	MET	SER	GLU	LYS	PHE	GLU	VAL	LYS	ASP	LEU
2	ASN	MET	LYS	PRO	GLY	MET	SER	LEU	LYS	ILE
3	LYS	GLY	LYS	ILE	HIS	ASN	ASP	VAL	ASP	ARG
4	PHE	LEU	ILE	ASN	LEU	GLY	GLN	GLY	LYS	GLU
5	THR	LEU	ASN	LEU	HIS	PHE	ASN	PRO	ARG	PHE
6	ASP	GLU	SER	THR	ILE	VAL	CYS	ASN	THR	SER
7	GLU	GLY	GLY	ARG	TRP	GLY	GLN	GLU	GLN	ARG
8	GLU	ASN	HIS	MET	CYS	PHE	SER	PRO	GLY	SER
9	GLU	VAL	LYS	ILE	THR	ILE	THR	PHE	GLN	ASP
10	LYS	ASP	PHE	LYS	VAL	THR	LEU	PRO	ASP	GLY
11	HIS	GLN	LEU	THR	PHE	PRO	ASN	ARG	LEU	GLY
12	HIS	ASN	GLN	LEU	HIS	TYR	LEU	SER	MET	GLY
13	GLY	LEU	GLN	ILE	SER	SER	PHE	LYS	LEU	GLU

1	MET	SER	GLU	LYS	PHE	GLU	VAL	LYS	ASP	LEU
2	ASN	MET	LYS	PRO	GLY	MET	SER	LEU	LYS	ILE
3	LYS	GLY	LYS	ILE	HIS	ASN	ASP	VAL	ASP	ARG
4	PHE	LEU	ILE	ASN	LEU	GLY	GLN	GLY	LYS	GLU
5	THR	LEU	ASN	LEU	HIS	PHE	ASN	PRO	ARG	PHE
6	ASP	GLU	SER	THR	ILE	VAL	CYS	ASN	THR	SER
7	GLU	GLY	GLY	ARG	TRP	GLY	GLN	GLU	GLN	ARG
8	GLU	ASN	HIS	MET	CYS	PHE	SER	PRO	GLY	SER
9	GLU	VAL	LYS	ILE	THR	ILE	THR	PHE	GLN	ASP
10	LYS	ASP	PHE	LYS	VAL	THR	LEU	PRO	ASP	GLY
11	HIS	GLN	LEU	THR	PHE	PRO	ASN	ARG	LEU	GLY
12	HIS	ASN	GLN	LEU	HIS	TYR	LEU	SER	MET	GLY
13	GLY	LEU	GLN	ILE	SER	SER	PHE	LYS	LEU	GLU

1	MET	SER	GLU	LYS	PHE	GLU	VAL	LYS	ASP	LEU
2	ASN	MET	LYS	PRO	GLY	MET	SER	LEU	LYS	ILE
3	LYS	GLY	LYS	ILE	HIS	ASN	ASP	VAL	ASP	ARG
4	PHE	LEU	ILE	ASN	LEU	GLY	GLN	GLY	LYS	GLU
5	THR	LEU	ASN	LEU	HIS	PHE	ASN	PRO	ARG	PHE
6	ASP	GLU	SER	THR	ILE	VAL	CYS	ASN	THR	SER
7	GLU	GLY	GLY	ARG	TRP	GLY	GLN	GLU	GLN	ARG
8	GLU	ASN	HIS	MET	CYS	PHE	SER	PRO	GLY	SER
9	GLU	VAL	LYS	ILE	THR	ILE	THR	PHE	GLN	ASP
10	LYS	ASP	PHE	LYS	VAL	THR	LEU	PRO	ASP	GLY
11	HIS	GLN	LEU	THR	PHE	PRO	ASN	ARG	LEU	GLY
12	HIS	ASN	GLN	LEU	HIS	TYR	LEU	SER	MET	GLY
13	GLY	LEU	GLN	ILE	SER	SER	PHE	LYS	LEU	GLU