BMRB Entry 27262

Name: '1H, 13C and 15N Chemical shift assignment for double stranded RNA Binding Domain 1 of Trans Activation Response element (TAR) RNA Binding protein 2'
Published: 2018-03-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27262

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: '1H, 13C and 15N Chemical shift assignment for double stranded RNA Binding Domain 1 of Trans Activation Response element (TAR) RNA Binding protein 2' PubMed: 29445910

Deposition date: 2017-09-23 Original release date: 2018-03-08

Authors: Chugh, Jeetender; Paithankar, Harshad; Sharma, Shilpy; Naglekar, Amit; Jadhav, Pankaj

Citation: Paithankar, Harshad; Jadhav, Pankaj; Naglekar, Amit; Sharma, Shilpy; Chugh, Jeetender. "1H, 13C and 15N resonance assignment of domain 1 of trans-activation response element (TAR) RNA binding protein isoform 1 (TRBP2) and its comparison with that of isoform 2 (TRBP1)" Biomol. NMR Assign. 12, 189-194 (2018).

Assembly members:
dsRBD1_of_TAR_RNA_Binding_Protein_2, polymer, 108 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSV272

Entity Sequences (FASTA):
dsRBD1_of_TAR_RNA_Binding_Protein_2: GGAMSEEEQGSGTTTGCGLP SIEQMLAANPGKTPISLLQE YGTRIGKTPVYDLLKAEGQA HQPNFTFRVTVGDTSCTGQG PSKKAAKHKAAEVALKHLKG GSMLEPAL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	225
15N chemical shifts	97
1H chemical shifts	290

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dsRBD1 of TAR RNA Binding Protein 2	1

Entities:

Entity 1, dsRBD1 of TAR RNA Binding Protein 2 108 residues - Formula weight is not available

1

GLY

ALA

MET

SER

GLU

GLN

GLY

2

SER

GLY

THR

GLY

CYS

GLY

LEU

PRO

3

SER

ILE

GLU

GLN

MET

LEU

ALA

ASN

PRO

4

GLY

LYS

THR

PRO

ILE

SER

LEU

GLN

GLU

5

TYR

GLY

THR

ARG

ILE

GLY

LYS

THR

PRO

VAL

6

TYR

ASP

LEU

LYS

ALA

GLU

GLY

GLN

ALA

7

HIS

GLN

PRO

ASN

PHE

THR

PHE

ARG

VAL

THR

8

VAL

GLY

ASP

THR

SER

CYS

THR

GLY

GLN

GLY

9

PRO

SER

LYS

ALA

LYS

HIS

LYS

ALA

10

ALA

GLU

VAL

ALA

LEU

LYS

HIS

LEU

LYS

GLY

11

GLY

SER

MET

LEU

GLU

PRO

ALA

LEU

Samples:

sample_1: dsRBD1 of TAR RNA Binding Protein 2, [U-100% 13C; U-100% 15N], 800 uM; sodium phosphate 10 mM; sodium chloride 100 mM; EDTA 1 mM; DTT 1 mM; D2O, [U-100% 2H], 5%

sample_2: dsRBD1 of TAR RNA Binding Protein 2, [U-100% 15N], 800 uM; sodium phosphate 10 mM; sodium chloride 100 mM; EDTA 1 mM; DTT 1 mM; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts