BMRB Entry 27268

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27268

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Title:
Backbone chemical shifts for the complex between WASp interacting protein (WIP) and the EVH1 domain of WASp
Deposition date:
2017-10-01
Original release date:
2017-12-14
Authors:
Halle-Bikovski, Adi; Shaked, Hadassa; Chill, Jordan
Citation:

Citation: Halle-Bikovski, Adi; Fried, Sophia; Rozentur-Shkop, Eva; Biber, Guy; Shaked, Hadassa; Joseph, Noah; Baarda-Saad, Mira; Chill, Jordan. "New Structural Insights into Formation of the Key Actin Regulating WIP-WASp Complex Determined by NMR and Molecular Imaging"  ACS Chem. Biol. 13, 100-109 (2018).
PubMed: 29215267

Assembly members:

Assembly members:
WIP(442-492), polymer, 51 residues, Formula weight is not available
WASp(20-158), polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
WIP(442-492): QDSPCEDEWESRFYFHPISD LPPPEPYVQTTKSYPSKLAR NESRSGSNRRE
WASp(20-158): QNIPSTLLQDHENQRLFEML GRKCLTLATAVVQLYLALPP GAEHWTKEHCGAVCFVKDNP QKSYFIRLYGLQAGRLLWEQ ELYSQLVYSTPTPFFHTFAG DDCQAGLNFADEDEAQAFRA LVQEKIQKRNQRQSGDRRQ

Data sets:
Data typeCount
13C chemical shifts526
15N chemical shifts167
1H chemical shifts563

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1WIP1
2WASp2

Entities:

Entity 1, WIP 51 residues - Formula weight is not available

1   GLNASPSERPROCYSGLUASPGLUTRPGLU
2   SERARGPHETYRPHEHISPROILESERASP
3   LEUPROPROPROGLUPROTYRVALGLNTHR
4   THRLYSSERTYRPROSERLYSLEUALAARG
5   ASNGLUSERARGSERGLYSERASNARGARG
6   GLU

Entity 2, WASp 139 residues - Formula weight is not available

1   GLNASNILEPROSERTHRLEULEUGLNASP
2   HISGLUASNGLNARGLEUPHEGLUMETLEU
3   GLYARGLYSCYSLEUTHRLEUALATHRALA
4   VALVALGLNLEUTYRLEUALALEUPROPRO
5   GLYALAGLUHISTRPTHRLYSGLUHISCYS
6   GLYALAVALCYSPHEVALLYSASPASNPRO
7   GLNLYSSERTYRPHEILEARGLEUTYRGLY
8   LEUGLNALAGLYARGLEULEUTRPGLUGLN
9   GLULEUTYRSERGLNLEUVALTYRSERTHR
10   PROTHRPROPHEPHEHISTHRPHEALAGLY
11   ASPASPCYSGLNALAGLYLEUASNPHEALA
12   ASPGLUASPGLUALAGLNALAPHEARGALA
13   LEUVALGLNGLULYSILEGLNLYSARGASN
14   GLNARGGLNSERGLYASPARGARGGLN

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks