BMRB Entry 27270

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27270

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Title:
Backbone assignments of NS5A-D2 from Hepatitis C virus (JFH-1) phosphorylated by Casein Kinase II
Deposition date:
2017-10-03
Original release date:
2017-10-31
Authors:
Hanoulle, Xavier
Citation:

Citation: Bessa, Luiza; Schneider, Robert; Hanoulle, Xavier. "NMR and circular dichroism data for domain 2 of the HCV NS5A protein phosphorylated by the Casein Kinase II"  Data Brief 17, .-325 (333).
PubMed: 29876401

Assembly members:

Assembly members:
pNS5A-D2, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hepatitis C   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7.7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
pNS5A-D2: MNTYDVDMVDANLLMEGGVA QTEPESRVPVLDFLEPMAEE EXDLEPSIPSECMLPRSGFP RALPAWARPDYNPPLVESWR RPDYQPPTVAGCALPLQHHH HHH

Data sets:
Data typeCount
13C chemical shifts278
15N chemical shifts80
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1pNS5A-D21

Entities:

Entity 1, pNS5A-D2 103 residues - Formula weight is not available

The first Methionine residue is a cloning artifact. The last 8 residues represent a non-native affinity tag. This is the domain 2 of the HCV (JFH1, genotype 2a) NS5A protein.

1   METASNTHRTYRASPVALASPMETVALASP
2   ALAASNLEULEUMETGLUGLYGLYVALALA
3   GLNTHRGLUPROGLUSERARGVALPROVAL
4   LEUASPPHELEUGLUPROMETALAGLUGLU
5   GLUSEPASPLEUGLUPROSERILEPROSER
6   GLUCYSMETLEUPROARGSERGLYPHEPRO
7   ARGALALEUPROALATRPALAARGPROASP
8   TYRASNPROPROLEUVALGLUSERTRPARG
9   ARGPROASPTYRGLNPROPROTHRVALALA
10   GLYCYSALALEUPROLEUGLNHISHISHIS
11   HISHISHIS

Related Database Links:

Swiss-Prot Q99IB8
GB AB047639

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks