BMRB Entry 27295
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27295
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Title: Methyl-methionine resonance assignments for Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, Isoprenaline-bound form PubMed: 29176642
Deposition date: 2017-10-26 Original release date: 2018-01-29
Authors: Solt, Andras; Bostock, Mark; Shrestha, Binesh; Kumar, Prashant; Warne, Tony; Tate, Christopher; Nietlispach, Daniel
Citation: Solt, Andras; Bostock, Mark; Shrestha, Binesh; Kumar, Prashant; Warne, Tony; Tate, Christopher; Nietlispach, Daniel. "Insight into partial agonism by observing multiple equilibria for ligand-bound and Gs-mimetic nanobody-bound beta1-adrenergic receptor" Nat. Commun. 8, 1795-1795 (2017).
Assembly members:
Turkey_B1AR_D5_L190M, polymer, 317 residues, Formula weight is not available
entity_5FW, non-polymer, 211.258 Da.
Natural source: Common Name: Turkey Taxonomy ID: 9103 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Meleagris gallopavo
Experimental source: Production method: recombinant technology Host organism: Spodoptera frugiperda Vector: pBacPAK8
Entity Sequences (FASTA):
Turkey_B1AR_D5_L190M: MGAELLSQQWEAGLSLLLAL
VVLLIVAGNVLVIAAIGSTQ
RLQTLTNLFITSLACADLVM
GLLVVPFGATLVVRGTWLWG
SFLCELWTSLDVLCVTASIW
TLCVIAIDRYLAITSPFRYQ
SLMTRARAKVIICTVWAISA
LVSFLPIMLHWWRDEDPQAM
KCYQDPGCCDFVTNRAYAIA
SSIISFYIPLLIMIFVYLRV
YREAKEQIRKIDRASKRKTS
RVAAMREHKALKTLGIIMGV
FTLCWLPFFLVNIVNVFNRD
LVPDWLFVAFNWLGYANSAA
NPIIYCRSPDFRKAFKRLLA
FPRKADRRLHHHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 14 |
| 1H chemical shifts | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 1 | 1 |
| 2 | Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 2 | 1 |
| 3 | Isoprenaline | 2 |
Entities:
Entity 1, Turkey beta-1 adrenergic receptor, Delta5-L190M mutant, conformer 1 317 residues - Formula weight is not available
| 1 | MET | GLY | ALA | GLU | LEU | LEU | SER | GLN | GLN | TRP | ||||
| 2 | GLU | ALA | GLY | LEU | SER | LEU | LEU | LEU | ALA | LEU | ||||
| 3 | VAL | VAL | LEU | LEU | ILE | VAL | ALA | GLY | ASN | VAL | ||||
| 4 | LEU | VAL | ILE | ALA | ALA | ILE | GLY | SER | THR | GLN | ||||
| 5 | ARG | LEU | GLN | THR | LEU | THR | ASN | LEU | PHE | ILE | ||||
| 6 | THR | SER | LEU | ALA | CYS | ALA | ASP | LEU | VAL | MET | ||||
| 7 | GLY | LEU | LEU | VAL | VAL | PRO | PHE | GLY | ALA | THR | ||||
| 8 | LEU | VAL | VAL | ARG | GLY | THR | TRP | LEU | TRP | GLY | ||||
| 9 | SER | PHE | LEU | CYS | GLU | LEU | TRP | THR | SER | LEU | ||||
| 10 | ASP | VAL | LEU | CYS | VAL | THR | ALA | SER | ILE | TRP | ||||
| 11 | THR | LEU | CYS | VAL | ILE | ALA | ILE | ASP | ARG | TYR | ||||
| 12 | LEU | ALA | ILE | THR | SER | PRO | PHE | ARG | TYR | GLN | ||||
| 13 | SER | LEU | MET | THR | ARG | ALA | ARG | ALA | LYS | VAL | ||||
| 14 | ILE | ILE | CYS | THR | VAL | TRP | ALA | ILE | SER | ALA | ||||
| 15 | LEU | VAL | SER | PHE | LEU | PRO | ILE | MET | LEU | HIS | ||||
| 16 | TRP | TRP | ARG | ASP | GLU | ASP | PRO | GLN | ALA | MET | ||||
| 17 | LYS | CYS | TYR | GLN | ASP | PRO | GLY | CYS | CYS | ASP | ||||
| 18 | PHE | VAL | THR | ASN | ARG | ALA | TYR | ALA | ILE | ALA | ||||
| 19 | SER | SER | ILE | ILE | SER | PHE | TYR | ILE | PRO | LEU | ||||
| 20 | LEU | ILE | MET | ILE | PHE | VAL | TYR | LEU | ARG | VAL | ||||
| 21 | TYR | ARG | GLU | ALA | LYS | GLU | GLN | ILE | ARG | LYS | ||||
| 22 | ILE | ASP | ARG | ALA | SER | LYS | ARG | LYS | THR | SER | ||||
| 23 | ARG | VAL | ALA | ALA | MET | ARG | GLU | HIS | LYS | ALA | ||||
| 24 | LEU | LYS | THR | LEU | GLY | ILE | ILE | MET | GLY | VAL | ||||
| 25 | PHE | THR | LEU | CYS | TRP | LEU | PRO | PHE | PHE | LEU | ||||
| 26 | VAL | ASN | ILE | VAL | ASN | VAL | PHE | ASN | ARG | ASP | ||||
| 27 | LEU | VAL | PRO | ASP | TRP | LEU | PHE | VAL | ALA | PHE | ||||
| 28 | ASN | TRP | LEU | GLY | TYR | ALA | ASN | SER | ALA | ALA | ||||
| 29 | ASN | PRO | ILE | ILE | TYR | CYS | ARG | SER | PRO | ASP | ||||
| 30 | PHE | ARG | LYS | ALA | PHE | LYS | ARG | LEU | LEU | ALA | ||||
| 31 | PHE | PRO | ARG | LYS | ALA | ASP | ARG | ARG | LEU | HIS | ||||
| 32 | HIS | HIS | HIS | HIS | HIS | HIS | HIS |
Entity 2, Isoprenaline - C11 H17 N O3 - 211.258 Da.
| 1 | 5FW |
Samples:
L190M-Isoprenaline: Turkey_B1AR_D5_L190M, 13Ce-Met, 90 uM; Isoprenaline 1 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 8.0; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C SOFAST-HMQC | L190M-Isoprenaline | isotropic | sample_conditions_1 |
Software:
CCPN_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz