BMRB Entry 27300
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27300
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Title: Backbone HN, N, CA, and CB assignment of human UBC9_P123L PubMed: 30574775
Deposition date: 2017-11-02 Original release date: 2019-01-10
Authors: Placzek, William
Citation: Wright, Christine; Whitaker, Robert; Onuiri, Joshua; Blackburn, Tessa; McGarity, Sierra; Bjornsti, Mary-Ann; Placzek, William. "UBC9 mutant reveals the impact of protein dynamics on substrate selectivity and SUMO chain linkages" Biochemistry 58, 621-632 (2019).
Assembly members:
UBC9(P123L), polymer, 178 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
UBC9(P123L): MGSSHHHHHHSSGLVPRGSH
MSGIALSRLAQERKAWRKDH
PFGFVAVPTKNPDGTMNLMN
WECAIPGKKGTPWEGGLFKL
RMLFKDDYPSSPPKCKFEPP
LFHPNVYPSGTVCLSILEED
KDWRPAITIKQILLGIQELL
NELNIQDPAQAEAYTIYCQN
RVEYEKRVRAQAKKFAPS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 295 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 129 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBC9_P123L monomer | 1 |
Entities:
Entity 1, UBC9_P123L monomer 178 residues - Formula weight is not available
Residues -19 to 0 are part of the His tag and were not assigned, 1 corresponds with the native residue M1.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | SER | GLY | ILE | ALA | LEU | SER | ARG | LEU | ALA | ||||
| 4 | GLN | GLU | ARG | LYS | ALA | TRP | ARG | LYS | ASP | HIS | ||||
| 5 | PRO | PHE | GLY | PHE | VAL | ALA | VAL | PRO | THR | LYS | ||||
| 6 | ASN | PRO | ASP | GLY | THR | MET | ASN | LEU | MET | ASN | ||||
| 7 | TRP | GLU | CYS | ALA | ILE | PRO | GLY | LYS | LYS | GLY | ||||
| 8 | THR | PRO | TRP | GLU | GLY | GLY | LEU | PHE | LYS | LEU | ||||
| 9 | ARG | MET | LEU | PHE | LYS | ASP | ASP | TYR | PRO | SER | ||||
| 10 | SER | PRO | PRO | LYS | CYS | LYS | PHE | GLU | PRO | PRO | ||||
| 11 | LEU | PHE | HIS | PRO | ASN | VAL | TYR | PRO | SER | GLY | ||||
| 12 | THR | VAL | CYS | LEU | SER | ILE | LEU | GLU | GLU | ASP | ||||
| 13 | LYS | ASP | TRP | ARG | PRO | ALA | ILE | THR | ILE | LYS | ||||
| 14 | GLN | ILE | LEU | LEU | GLY | ILE | GLN | GLU | LEU | LEU | ||||
| 15 | ASN | GLU | LEU | ASN | ILE | GLN | ASP | PRO | ALA | GLN | ||||
| 16 | ALA | GLU | ALA | TYR | THR | ILE | TYR | CYS | GLN | ASN | ||||
| 17 | ARG | VAL | GLU | TYR | GLU | LYS | ARG | VAL | ARG | ALA | ||||
| 18 | GLN | ALA | LYS | LYS | PHE | ALA | PRO | SER |
Samples:
sample_1: UBC9(P123L), [U-99% 13C; U-99% 15N], 500 uM; sodium phosphate 20 mM; sodium chloride 120 mM; D2O 5%; Glutamic acid 10 mM
sample_conditions_1: ionic strength: 0.14 M; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts