BMRB Entry 27301
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27301
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Title: Backbone and side chain assignments of Scm3 from Saccharomyces cerevisiae PubMed: 30937734
Deposition date: 2017-11-07 Original release date: 2019-04-12
Authors: Bhattacharya, Anusri; Shukla, Vaibhav; Hosur, Ramakrishna; Kumar, Ashutosh
Citation: Bhattacharya, Anusri; Shukla, Vaibhav; Hosur, Ramakrishna; Kumar, Ashutosh. "Backbone and side-chain resonance assignments of centromeric protein Scm3 from Saccharomyces cerevisiae" Biomol. NMR Assign. 13, 267-273 (2019).
Assembly members:
Scm3, polymer, 223 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHAT2
Entity Sequences (FASTA):
Scm3: MKTNKKISKRRSLKNLHGAL
KGLLKESGKKSESKIRKHSD
CNPVHRVYPPNIEKRKTKKD
DGISRPIAERNGHVYIMSKE
NHIIPKLTDDEVMERHKLAD
ENMRKVWSNIISKYESIEEQ
GDLVDLKTGEIVEDNGHIKT
LTANNSTKDKRTKYTSVLRD
IIDISDEEDGDKNDEYTLWA
NDSEASDSEVDADNDTEEEK
DEKLIDADFKKYEAKLSKRI
LRD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 754 |
| 15N chemical shifts | 203 |
| 1H chemical shifts | 1247 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Scm3 | 1 |
Entities:
Entity 1, Scm3 223 residues - Formula weight is not available
| 1 | MET | LYS | THR | ASN | LYS | LYS | ILE | SER | LYS | ARG | ||||
| 2 | ARG | SER | LEU | LYS | ASN | LEU | HIS | GLY | ALA | LEU | ||||
| 3 | LYS | GLY | LEU | LEU | LYS | GLU | SER | GLY | LYS | LYS | ||||
| 4 | SER | GLU | SER | LYS | ILE | ARG | LYS | HIS | SER | ASP | ||||
| 5 | CYS | ASN | PRO | VAL | HIS | ARG | VAL | TYR | PRO | PRO | ||||
| 6 | ASN | ILE | GLU | LYS | ARG | LYS | THR | LYS | LYS | ASP | ||||
| 7 | ASP | GLY | ILE | SER | ARG | PRO | ILE | ALA | GLU | ARG | ||||
| 8 | ASN | GLY | HIS | VAL | TYR | ILE | MET | SER | LYS | GLU | ||||
| 9 | ASN | HIS | ILE | ILE | PRO | LYS | LEU | THR | ASP | ASP | ||||
| 10 | GLU | VAL | MET | GLU | ARG | HIS | LYS | LEU | ALA | ASP | ||||
| 11 | GLU | ASN | MET | ARG | LYS | VAL | TRP | SER | ASN | ILE | ||||
| 12 | ILE | SER | LYS | TYR | GLU | SER | ILE | GLU | GLU | GLN | ||||
| 13 | GLY | ASP | LEU | VAL | ASP | LEU | LYS | THR | GLY | GLU | ||||
| 14 | ILE | VAL | GLU | ASP | ASN | GLY | HIS | ILE | LYS | THR | ||||
| 15 | LEU | THR | ALA | ASN | ASN | SER | THR | LYS | ASP | LYS | ||||
| 16 | ARG | THR | LYS | TYR | THR | SER | VAL | LEU | ARG | ASP | ||||
| 17 | ILE | ILE | ASP | ILE | SER | ASP | GLU | GLU | ASP | GLY | ||||
| 18 | ASP | LYS | ASN | ASP | GLU | TYR | THR | LEU | TRP | ALA | ||||
| 19 | ASN | ASP | SER | GLU | ALA | SER | ASP | SER | GLU | VAL | ||||
| 20 | ASP | ALA | ASP | ASN | ASP | THR | GLU | GLU | GLU | LYS | ||||
| 21 | ASP | GLU | LYS | LEU | ILE | ASP | ALA | ASP | PHE | LYS | ||||
| 22 | LYS | TYR | GLU | ALA | LYS | LEU | SER | LYS | ARG | ILE | ||||
| 23 | LEU | ARG | ASP |
Samples:
sample_1: sodium phosphate 20 mM; sodium chloride 500 mM; DTT 5 mM; Scm3, [U-15N], 1 mM
sample_2: sodium phosphate 20 mM; sodium chloride 500 mM; DTT 5 mM; Scm3, [U-13C; U-15N], 1 mM
sample_conditions_1: ionic strength: 500 mM; pH: 7.4; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCcoNH | sample_2 | isotropic | sample_conditions_1 |
| 3D CCcoNH | sample_2 | isotropic | sample_conditions_1 |
| 3D HNN | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D MUSIC | sample_2 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker ASCEND 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts