BMRB Entry 27302

Name: 1H, 13C and 15N backbone chemical shift assignments of A. thaliana RCD1(499-572)
Published: 2018-04-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27302

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C and 15N backbone chemical shift assignments of A. thaliana RCD1(499-572) PubMed: 29657132

Deposition date: 2017-11-07 Original release date: 2018-04-13

Authors: Staby, Lasse; Kragelund, Birthe; Bugge, Katrine; Skriver, Karen

Citation: Bugge, Katrine; Staby, Lasse; Kemplen, Katherine; O'Shea, Charlotte; Bendsen, Sidsel; Jensen, Michael; Olsen, Johan; Skriver, Karen; Kragelund, Birthe. "Structure of Radical-induced Cell Death1 hub domain reveals a common aa-scaffold for disorder in transcriptional networks" Structure 26, 734-746 (2018).

Assembly members:
Radical-Induced_Cell_Death1, polymer, 75 residues, Formula weight is not available

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11a

Entity Sequences (FASTA):
Radical-Induced_Cell_Death1: MSTTRPKSPWMPFPTLFAAI SHKVAENDMLLINADYQQLR DKKMTRAEFVRKLRVIVGDD LLRSTITTLQNQPKS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	199
15N chemical shifts	64
1H chemical shifts	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Radical-Induced Cell Death1	1

Entities:

Entity 1, Radical-Induced Cell Death1 75 residues - Formula weight is not available

1

MET

SER

THR

ARG

PRO

LYS

SER

PRO

TRP

2

MET

PRO

PHE

PRO

THR

LEU

PHE

ALA

ILE

3

SER

HIS

LYS

VAL

ALA

GLU

ASN

ASP

MET

LEU

4

LEU

ILE

ASN

ALA

ASP

TYR

GLN

LEU

ARG

5

ASP

LYS

MET

THR

ARG

ALA

GLU

PHE

VAL

6

ARG

LYS

LEU

ARG

VAL

ILE

VAL

GLY

ASP

7

LEU

ARG

SER

THR

ILE

THR

LEU

GLN

8

ASN

GLN

PRO

LYS

SER

Samples:

sample_1: Radical-Induced Cell Death1, [U-100% 13C; U-100% 15N], 250 uM; potassium phosphate 20 mM; sodium chloride 100 mM; sodium azide 0.02 % w/v; DSS 0.7 mM; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

CCPN_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking

TOPSPIN v3.5, Bruker Biospin - collection

NMRDraw v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

MddNMR v2.5, Orekhov, Jaravine, Mayzel, Kazimierczuk - processing

NMR spectrometers:

Bruker Avance 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts