BMRB Entry 27303
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27303
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Title: 1H, 13C and 15N backbone chemical shift assignments of A. thaliana DREB2A(255-272) PubMed: 29657132
Deposition date: 2017-11-07 Original release date: 2018-04-13
Authors: Staby, Lasse; Skriver, Karen; Kragelund, Birthe
Citation: Bugge, Katrine; Staby, Lasse; Kemplen, Katherine; O'Shea, Charlotte; Bendsen, Sidsel; Jensen, Michael; Olsen, Johan; Skriver, Karen; Kragelund, Birthe. "Structure of Radical-induced Cell Death1 hub domain reveals a common aa-scaffold for disorder in transcriptional networks" Structure 26, 734-746 (2018).
Assembly members:
DREB2A(255-272), polymer, 19 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-4T-1
Entity Sequences (FASTA):
DREB2A(255-272): GSSDMFDVDELLRDLNGDD
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 55 |
15N chemical shifts | 20 |
1H chemical shifts | 21 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | DREB2A | 1 |
Entities:
Entity 1, DREB2A 19 residues - Formula weight is not available
N-terminal glycine from TEV cleavage site
1 | GLY | SER | SER | ASP | MET | PHE | ASP | VAL | ASP | GLU | ||||
2 | LEU | LEU | ARG | ASP | LEU | ASN | GLY | ASP | ASP |
Samples:
sample_1: DREB2A, [U-100% 13C; U-100% 15N], 150 uM; sodium chloride 100 mM; DSS 0.7 mM; D2O 10%; PMSF 0.1 mM; sodium azide 0.02 % w/v
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CCPN_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking
NMRDraw v8.9, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Mdd_NMR v2.5, Orekhov, Jaravine, Mayzel, Kazimierczuk - processing
TOPSPIN v3.5, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 750 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts