BMRB Entry 27306
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27306
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for FASP peptide of mPER2 PubMed: 29784789
Deposition date: 2017-11-07 Original release date: 2018-06-01
Authors: Partch, Carrie; Hunt, Sabrina; Narasimamurthy, Rajesh; Lu, Yining; Fustin, Jean-Michel; Okamura, Hitoshi; Kim, Jae Kyoung; Forger, Daniel; Virshup, David
Citation: Narasimamurthy, Rajesh; Hunt, Sabrina; Lu, Yining; Fustin, Jean-Michel; Okamura, Hitoshi; Partch, Carrie; Forger, Daniel; Kim, Jae Kyoung; Virshup, David. "CK1delta/epsilon protein kinase primes the PER2 circadian phosphoswitch." Proc. Natl. Acad. Sci. U.S.A. 115, 5986-5991 (2018).
Assembly members:
PER2_FASP, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b
Entity Sequences (FASTA):
PER2_FASP: GAMDPEFWRKKKTEVSAHLS
SLTLPGKAESVVSLTSQ
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 100 |
15N chemical shifts | 31 |
1H chemical shifts | 31 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | mPER2 FASP | 1 |
Entities:
Entity 1, mPER2 FASP 37 residues - Formula weight is not available
Residues 1-12 represent non-native sequence. Residues 13-37 map to mPER2 residues 642-666.
1 | GLY | ALA | MET | ASP | PRO | GLU | PHE | TRP | ARG | LYS | ||||
2 | LYS | LYS | THR | GLU | VAL | SER | ALA | HIS | LEU | SER | ||||
3 | SER | LEU | THR | LEU | PRO | GLY | LYS | ALA | GLU | SER | ||||
4 | VAL | VAL | SER | LEU | THR | SER | GLN |
Samples:
sample_1: PER2 FASP, [U-13C; U-15N], 0.4 mM; MOPS 25 mM; sodium chloride 50 mM; EDTA 1 mM; Pierce protease inhibitor tablet 1 tablet/50mL; D2O, [U-2H], 10%
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CcpNMR_Analysis v2.4.2, CCPN - chemical shift assignment, data analysis, peak picking
hmsIST, Hyberts, Takeuchi, Wagner - data reconstruction
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts