BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules 		Member of WWPDB

BMRB Entry 27307

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27307

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Title: NMR resonance assignments of RNase P protein from Thermotoga maritima   PubMed: 29450823

Deposition date: 2017-11-08 Original release date: 2018-03-08

Authors: Zeng, Danyun; Reiter, Nicholas

Citation: Zeng, Danyun; Brown, Ben; Voehler, Markus; Cai, Sheng; Reiter, Nicholas. "NMR resonance assignments of RNase P protein from Thermotoga maritima"  Biomol. NMR Assign. 12, 183-187 (2018).

Assembly members:
RNase_P_protein_from_Thermotoga_maritima, polymer, 118 residues, Formula weight is not available

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX4Ta

Entity Sequences (FASTA):
RNase_P_protein_from_Thermotoga_maritima: GSTESFTRRERLRLRRDFLL IFKEGKSLQNEYFVVLFRKN GLDYSRLGIVVKRKFGKATR RNKLKRWVREIFRRNKGVIP KGFDIVVIPRKKLSEEFERV DFWTVREKLLNLLKRIEG

Data sets:
Data typeCount
13C chemical shifts547
15N chemical shifts115
1H chemical shifts871

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNase P protein monomer1

Entities:

Entity 1, RNase P protein monomer 118 residues - Formula weight is not available

1   GLYSERTHRGLUSERPHETHRARGARGGLU
2   ARGLEUARGLEUARGARGASPPHELEULEU
3   ILEPHELYSGLUGLYLYSSERLEUGLNASN
4   GLUTYRPHEVALVALLEUPHEARGLYSASN
5   GLYLEUASPTYRSERARGLEUGLYILEVAL
6   VALLYSARGLYSPHEGLYLYSALATHRARG
7   ARGASNLYSLEULYSARGTRPVALARGGLU
8   ILEPHEARGARGASNLYSGLYVALILEPRO
9   LYSGLYPHEASPILEVALVALILEPROARG
10   LYSLYSLEUSERGLUGLUPHEGLUARGVAL
11   ASPPHETRPTHRVALARGGLULYSLEULEU
12   ASNLEULEULYSARGILEGLUGLY

Samples:

sample_1: RNase P protein from Thermotoga maritima, [U-100% 13C; U-100% 15N], 400 uM; sodium phosphate 20 mM; sodium chloride 80 mM; DSS 50 uM; D2O, [U-99% 2H], 5 % v/v

sample_2: RNase P protein from Thermotoga maritima, [U-100% 13C; U-100% 15N], 400 uM; sodium phosphate 20 mM; sodium chloride 80 mM; DSS 50 uM; D2O 100 % v/v

sample_conditions_1: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1

Software:

CARA v1.9.1.4, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz
  • Varian INOVA 600 MHz

Related Database Links:

UNP Q9X1H4
AlphaFold Q9X1H4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts