BMRB Entry 27330
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27330
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NMR-STAR v3 text file.
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Citation: Zhang, Nan; Liu, Jing; Liu, Yajie; Wu, Wen-Hao; Fang, Jing; Da, Xiao-Di; Wang, Shenlin; Zhang, Wen-Bin. "NMR Spectroscopic Studies Reveal the Critical Role of the Isopeptide Bond in Forming the Otherwise Unstable SpyTag-SpyCatcher Mutant Complexes" Biochemistry 59, 2226-2236 (2020).
PubMed: 32469203
Assembly members:
SpyCatcher, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: Streptococcus pyogenes Taxonomy ID: 1314 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus pyogenes
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a
Entity Sequences (FASTA):
SpyCatcher: AMVDTLSGLSSEQGQSGDMT
IEEDSATHIKFSKRDEDGKE
LAGATMELRDSSGKTISTWI
SDGQVKDFYLYPGKYTFVET
AAPDGYEVATAITFTVNEQG
QVTVNGKATKGDAH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 260 |
| 15N chemical shifts | 93 |
| 1H chemical shifts | 93 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SpyCatcher | 1 |
Entities:
Entity 1, SpyCatcher 114 residues - Formula weight is not available
| 1 | ALA | MET | VAL | ASP | THR | LEU | SER | GLY | LEU | SER | ||||
| 2 | SER | GLU | GLN | GLY | GLN | SER | GLY | ASP | MET | THR | ||||
| 3 | ILE | GLU | GLU | ASP | SER | ALA | THR | HIS | ILE | LYS | ||||
| 4 | PHE | SER | LYS | ARG | ASP | GLU | ASP | GLY | LYS | GLU | ||||
| 5 | LEU | ALA | GLY | ALA | THR | MET | GLU | LEU | ARG | ASP | ||||
| 6 | SER | SER | GLY | LYS | THR | ILE | SER | THR | TRP | ILE | ||||
| 7 | SER | ASP | GLY | GLN | VAL | LYS | ASP | PHE | TYR | LEU | ||||
| 8 | TYR | PRO | GLY | LYS | TYR | THR | PHE | VAL | GLU | THR | ||||
| 9 | ALA | ALA | PRO | ASP | GLY | TYR | GLU | VAL | ALA | THR | ||||
| 10 | ALA | ILE | THR | PHE | THR | VAL | ASN | GLU | GLN | GLY | ||||
| 11 | GLN | VAL | THR | VAL | ASN | GLY | LYS | ALA | THR | LYS | ||||
| 12 | GLY | ASP | ALA | HIS |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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