BMRB Entry 27339
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27339
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Title: Chemical shifts of UBQLN2 residues 450-624 PubMed: 29526694
Deposition date: 2017-12-11 Original release date: 2018-03-16
Authors: Castaneda, Carlos
Citation: Dao, Thuy; Kolaitis, Regina-Maria; Kim, Hong Joo; O'Donovan, Kevin; Martyniak, Brian; Colicino, Erica; Hehnly, Heidi; Taylor, J Paul; Castaneda, Carlos. "Ubiquitin Modulates Liquid-Liquid Phase Separation of UBQLN2 via Disruption of Multivalent Interactions" Mol. Cell S1097, 30102-30103 (2018).
Assembly members:
UBQLN2_450-624, polymer, 177 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24b
Entity Sequences (FASTA):
UBQLN2_450-624: MRAMQALMQIQQGLQTLATE
APGLIPSFTPGVGVGVLGTA
IGPVGPVTPIGPIGPIVPFT
PIGPIGPIGPTGPAAPPGST
GSGGPTGPTVSSAAPSETTS
PTSESGPNQQFIQQMVQALA
GANAPQLPNPEVRFQQQLEQ
LNAMGFLNREANLQALIATG
GDINAAIERLLGSQPSW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 435 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 132 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBQLN2 | 1 |
Entities:
Entity 1, UBQLN2 177 residues - Formula weight is not available
Second residue R is residue 450 of human UBQLN2. Additional C-terminal residue W for prot concentration measurements.
| 1 | MET | ARG | ALA | MET | GLN | ALA | LEU | MET | GLN | ILE | ||||
| 2 | GLN | GLN | GLY | LEU | GLN | THR | LEU | ALA | THR | GLU | ||||
| 3 | ALA | PRO | GLY | LEU | ILE | PRO | SER | PHE | THR | PRO | ||||
| 4 | GLY | VAL | GLY | VAL | GLY | VAL | LEU | GLY | THR | ALA | ||||
| 5 | ILE | GLY | PRO | VAL | GLY | PRO | VAL | THR | PRO | ILE | ||||
| 6 | GLY | PRO | ILE | GLY | PRO | ILE | VAL | PRO | PHE | THR | ||||
| 7 | PRO | ILE | GLY | PRO | ILE | GLY | PRO | ILE | GLY | PRO | ||||
| 8 | THR | GLY | PRO | ALA | ALA | PRO | PRO | GLY | SER | THR | ||||
| 9 | GLY | SER | GLY | GLY | PRO | THR | GLY | PRO | THR | VAL | ||||
| 10 | SER | SER | ALA | ALA | PRO | SER | GLU | THR | THR | SER | ||||
| 11 | PRO | THR | SER | GLU | SER | GLY | PRO | ASN | GLN | GLN | ||||
| 12 | PHE | ILE | GLN | GLN | MET | VAL | GLN | ALA | LEU | ALA | ||||
| 13 | GLY | ALA | ASN | ALA | PRO | GLN | LEU | PRO | ASN | PRO | ||||
| 14 | GLU | VAL | ARG | PHE | GLN | GLN | GLN | LEU | GLU | GLN | ||||
| 15 | LEU | ASN | ALA | MET | GLY | PHE | LEU | ASN | ARG | GLU | ||||
| 16 | ALA | ASN | LEU | GLN | ALA | LEU | ILE | ALA | THR | GLY | ||||
| 17 | GLY | ASP | ILE | ASN | ALA | ALA | ILE | GLU | ARG | LEU | ||||
| 18 | LEU | GLY | SER | GLN | PRO | SER | TRP |
Samples:
13C_15N_450C: UBQLN2 450-624, [U-99% 13C; U-99% 15N], 200 uM; Na Phosphate 20 mM; EDTA 0.1 mM
buffer_conditions: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | 13C_15N_450C | isotropic | buffer_conditions |
| 3D HNCACB | 13C_15N_450C | isotropic | buffer_conditions |
| 3D CBCA(CO)NH | 13C_15N_450C | isotropic | buffer_conditions |
| 3D HNCA | 13C_15N_450C | isotropic | buffer_conditions |
| 3D HN(CA)CO | 13C_15N_450C | isotropic | buffer_conditions |
| 2D 1H-15N HSQC | 13C_15N_450C | isotropic | buffer_conditions |
| 2D N-CO (C-detect) | 13C_15N_450C | isotropic | buffer_conditions |
| 3D HACANCACON (C-detect) | 13C_15N_450C | isotropic | buffer_conditions |
| 3D HACANCANCO (C-detect) | 13C_15N_450C | isotropic | buffer_conditions |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CCPNMR, CCPN - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts