BMRB Entry 27342

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27342

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone assignments for the N-terminal domain of VirB10
Deposition date:
2017-12-19
Original release date:
2018-10-23
Authors:
Oliveira, Luciana; Salinas, Roberto; Farah, Chuck
Citation:

Citation: Sgro, German; Costa, Tiago; Cenens, Willian; Souza, Diorge; Cassago, Alexandre; Oliveira, Luciana; Salinas, Roberto; Portugal, Rodrigo; Farah, Chuck; Waksman, Gabriel. "Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri"  Nat. Microbiol. 3, 1429-1440 (2018).
PubMed: 30349081

Assembly members:

Assembly members:
VirB10NT, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Xanthomonas citri   Taxonomy ID: 346   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Xanthomonas citri

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28(a)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
VirB10NT: GSHMQSGEDSAPPKPRTETV VAPALPQSMTAPVEEAPVPL AQQPSLPPLPPMPTDNSEEV SSAPERQRGPTLLERRILAE SAANGGGVPGQLGAQPAPTQ ED

Data sets:
Data typeCount
13C chemical shifts373
15N chemical shifts82
1H chemical shifts82
heteronuclear NOE values62

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VirB10NT1

Entities:

Entity 1, VirB10NT 102 residues - Formula weight is not available

The 4 first Residues (GSHM) represent a reminiscent non-native fragment from the affinity tag.

1   GLYSERHISMETGLNSERGLYGLUASPSER
2   ALAPROPROLYSPROARGTHRGLUTHRVAL
3   VALALAPROALALEUPROGLNSERMETTHR
4   ALAPROVALGLUGLUALAPROVALPROLEU
5   ALAGLNGLNPROSERLEUPROPROLEUPRO
6   PROMETPROTHRASPASNSERGLUGLUVAL
7   SERSERALAPROGLUARGGLNARGGLYPRO
8   THRLEULEUGLUARGARGILELEUALAGLU
9   SERALAALAASNGLYGLYGLYVALPROGLY
10   GLNLEUGLYALAGLNPROALAPROTHRGLN
11   GLUASP

Related Database Links:

REF WP_005914234.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks