BMRB Entry 27354

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PDB ID: 5zcz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27354
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments for the Thermus thermophilus HB8 TTHA1718 protein in sf9 cells by in-cell NMR spectroscopy
Deposition date:
2018-01-10
Original release date:
2020-05-29
Authors:
Ito, Yutaka; Ikeya, Teppei; Tanaka, Takashi; Kamoshida, Hajime; Mishima, Masaki; Shirakawa, Masahiro; Guentert, Peter
Citation:

Citation: Tanaka, Takashi; Ikeya, Teppei; Kamoshida, Hajime; Suemoto, Yusuke; Mishima, Masaki; Shirakawa, Masahiro; Guntert, Peter; Ito, Yutaka. "High-Resolution Protein 3D Structure Determination in Living Eukaryotic Cells"  Angew. Chem. Int. Ed. Engl. 58, 7284-7288 (2019).
PubMed: 30938016

Assembly members:

Assembly members:
TTHA1718, polymer, 66 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Spodoptera frugiperda   Vector: pFastBac

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TTHA1718: MLKLKVEGMTCNHCVMAVTK ALKKVPGVEKVEVSLEKGEA LVEGTADPKALVQAVEEEGY KAEVLA

Data sets:
Data typeCount
13C chemical shifts89
15N chemical shifts56
1H chemical shifts287

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1the Thermus thermophilus HB8 TTHA1718 protein1

Entities:

Entity 1, the Thermus thermophilus HB8 TTHA1718 protein 66 residues - Formula weight is not available

1   METLEULYSLEULYSVALGLUGLYMETTHR
2   CYSASNHISCYSVALMETALAVALTHRLYS
3   ALALEULYSLYSVALPROGLYVALGLULYS
4   VALGLUVALSERLEUGLULYSGLYGLUALA
5   LEUVALGLUGLYTHRALAASPPROLYSALA
6   LEUVALGLNALAVALGLUGLUGLUGLYTYR
7   LYSALAGLUVALLEUALA

Related Database Links:

BMRB 11035 11037

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