BMRB Entry 27365
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR27365
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Title: CSP1_DPC PubMed: 30125091
Deposition date: 2018-01-18 Original release date: 2019-02-08
Authors: Yang, Yifang; Cornilescu, Gabriel; Tal-Gan, Yftah
Citation: Yang, Yifang; Cornilescu, Gabriel; Tal-Gan, Yftah. "Structural Characterization of Competence-Stimulating Peptide Analogues Reveals Key Features for ComD1 and ComD2 Receptor Binding in Streptococcus pneumoniae" Biochemistry 57, 5359-5369 (2018).
Assembly members:
CSP1, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: Streptococcus pneumoniae Taxonomy ID: 1313 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus pneumoniae
Experimental source: Production method: chemical synthesis Host organism: streptococcus pneumoniae
Entity Sequences (FASTA):
CSP1: EMRLSKFFRDFILQRKK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 32 |
15N chemical shifts | 18 |
1H chemical shifts | 129 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | CSP1, 1 | 1 |
2 | CSP1, 2 | 1 |
Entities:
Entity 1, CSP1, 1 17 residues - Formula weight is not available
1 | GLU | MET | ARG | LEU | SER | LYS | PHE | PHE | ARG | ASP | ||||
2 | PHE | ILE | LEU | GLN | ARG | LYS | LYS |
Samples:
sample_1: DPC, [U-2H], 250 mM; CSP1 1.9 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
X-PLOR_NIH vv. 2.42, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution
NMR spectrometers:
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts