BMRB Entry 27416
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27416
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Title: Backbone 1H, 15N, 13C assignment of Human Myc S373D/T400D, N353 to A399. PubMed: 29695509
Deposition date: 2018-03-06 Original release date: 2018-04-11
Authors: Macek, Pavel
Citation: Macek, Pavel; Cliff, Matthew; Embrey, Kevin; Holdgate, Geoffrey; Nissink, J Willem; Panova, Stanislava; Waltho, Jonathan; Davies, Rick. "Myc phosphorylation in its basic helix-loop-helix region destabilizes transient alpha-helical structures, disrupting Max and DNA binding" J. Biol. Chem. 293, 9301-9310 (2018).
Assembly members:
cMyc_S373D-T400D, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
cMyc_S373D-T400D: GSNVKRRTHNVLERQRRNEL
KRDFFALRDQIPELENNEKA
PKVVILKKADAYILSVQAEE
QKLISEEDLLRKRREQLKHK
LEQLRNS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 94 |
| 15N chemical shifts | 45 |
| 1H chemical shifts | 45 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cMyc S373D/T400D | 1 |
Entities:
Entity 1, cMyc S373D/T400D 87 residues - Formula weight is not available
| 1 | GLY | SER | ASN | VAL | LYS | ARG | ARG | THR | HIS | ASN | ||||
| 2 | VAL | LEU | GLU | ARG | GLN | ARG | ARG | ASN | GLU | LEU | ||||
| 3 | LYS | ARG | ASP | PHE | PHE | ALA | LEU | ARG | ASP | GLN | ||||
| 4 | ILE | PRO | GLU | LEU | GLU | ASN | ASN | GLU | LYS | ALA | ||||
| 5 | PRO | LYS | VAL | VAL | ILE | LEU | LYS | LYS | ALA | ASP | ||||
| 6 | ALA | TYR | ILE | LEU | SER | VAL | GLN | ALA | GLU | GLU | ||||
| 7 | GLN | LYS | LEU | ILE | SER | GLU | GLU | ASP | LEU | LEU | ||||
| 8 | ARG | LYS | ARG | ARG | GLU | GLN | LEU | LYS | HIS | LYS | ||||
| 9 | LEU | GLU | GLN | LEU | ARG | ASN | SER |
Samples:
sample_1: cMyc S373D/T400D, [U-100% 13C; U-100% 15N], 0.15 mM; potassium phosphate 50 mM; ammonium chloride 500 mM; DTT 1 mM; EDTA 1 mM
sample_conditions_1: ionic strength: 550 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CCPNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts