BMRB Entry 27427

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27427

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Title:
Methyl assignment of human eukaryotic translation initiation factor 4E (eIF4E) in its apo form
Deposition date:
2018-03-18
Original release date:
2022-05-10
Authors:
Volpon, Laurent; Osborne, Michael; Borden, Katherine
Citation:

Citation: Osborne, Michael; Volpon, Laurent; Memarpoor-Yazdi, Mina; Pillay, Shubhadra; Thambipillai, Aksharh; Czarnota, Sylwia; Culjkovic-Kraljacic, Biljana; Trahan, Christian; Oeffinger, Marlene; Cowling, Victoria; Borden, Katherine. "Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E"  J. Mol. Biol. 434, 167451-167451 (2022).
PubMed: 35026230

Assembly members:

Assembly members:
eIF4E, polymer, 217 residues, 25097.25 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF4E: MATVEPETTPTPNPPTTEEE KTESNQEVANPEHYIKHPLQ NRWALWFFKNDKSKTWQANL RLISKFDTVEDFWALYNHIQ LSSNLMPGCDYSLFKDGIEP MWEDEKNKRGGRWLITLNKQ QRRSDLDRFWLETLLCLIGE SFDDYSDDVCGAVVNVRAKG DKIAIWTTECENREAVTHIG RVYKERLGLPPKIVIGYQSH ADTATKSGSTTKNRFVV

Data sets:
Data typeCount
13C chemical shifts171
15N chemical shifts207
1H chemical shifts411

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer eIF4E1

Entities:

Entity 1, monomer eIF4E 217 residues - 25097.25 Da.

1   METALATHRVALGLUPROGLUTHRTHRPRO
2   THRPROASNPROPROTHRTHRGLUGLUGLU
3   LYSTHRGLUSERASNGLNGLUVALALAASN
4   PROGLUHISTYRILELYSHISPROLEUGLN
5   ASNARGTRPALALEUTRPPHEPHELYSASN
6   ASPLYSSERLYSTHRTRPGLNALAASNLEU
7   ARGLEUILESERLYSPHEASPTHRVALGLU
8   ASPPHETRPALALEUTYRASNHISILEGLN
9   LEUSERSERASNLEUMETPROGLYCYSASP
10   TYRSERLEUPHELYSASPGLYILEGLUPRO
11   METTRPGLUASPGLULYSASNLYSARGGLY
12   GLYARGTRPLEUILETHRLEUASNLYSGLN
13   GLNARGARGSERASPLEUASPARGPHETRP
14   LEUGLUTHRLEULEUCYSLEUILEGLYGLU
15   SERPHEASPASPTYRSERASPASPVALCYS
16   GLYALAVALVALASNVALARGALALYSGLY
17   ASPLYSILEALAILETRPTHRTHRGLUCYS
18   GLUASNARGGLUALAVALTHRHISILEGLY
19   ARGVALTYRLYSGLUARGLEUGLYLEUPRO
20   PROLYSILEVALILEGLYTYRGLNSERHIS
21   ALAASPTHRALATHRLYSSERGLYSERTHR
22   THRLYSASNARGPHEVALVAL

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