BMRB Entry 27460

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27460

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Title:
chemical shift assignnment of recombinant repetitive domain of spider dragline silk
Deposition date:
2018-04-23
Original release date:
2018-04-27
Authors:
Oktaviani, Nur; Matsugami, Akimasa; Malay, Ali; Hayashi, Fumiaki; Kaplan, David; Numata, Keiji
Citation:

Citation: Oktaviani, Nur; Matsugami, Akimasa; Malay, Ali; Hayashi, Fumiaki; Kaplan, David; Numata, Keiji. "Conformation and dynamics of soluble repetitive domain elucidates the initial beta-sheet formation of spider silk"  Nat. Commun. 9, 2121-2121 (2018).
PubMed: 29844575

Assembly members:

Assembly members:
repetitive_domain_spider_dragline_silk, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: spider   Taxonomy ID: 6915   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Nephila clavipes

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
repetitive_domain_spider_dragline_silk: MHHHHHHSSGLVPRGSGMKE TAAAKFERQHMDSPDLGTDD DDKAMAASGRGGLGGQGAGA AAAAGGAGQGGYGGLGSQGT SG

Data typeCount
13C chemical shifts860
15N chemical shifts309
1H chemical shifts1166

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1repetitive domain spider dragline silk monomer1
2repetitive domain spider dragline silk dimer1
3repetitive domain spider dragline silk trimer1
4repetitive domain spider dragline silk hexamer1
5repetitive domain spider dragline silk 15-mer1

Entities:

Entity 1, repetitive domain spider dragline silk monomer 82 residues - Formula weight is not available

1   METHISHISHISHISHISHISSERSERGLY
2   LEUVALPROARGGLYSERGLYMETLYSGLU
3   THRALAALAALALYSPHEGLUARGGLNHIS
4   METASPSERPROASPLEUGLYTHRASPASP
5   ASPASPLYSALAMETALAALASERGLYARG
6   GLYGLYLEUGLYGLYGLNGLYALAGLYALA
7   ALAALAALAALAGLYGLYALAGLYGLNGLY
8   GLYTYRGLYGLYLEUGLYSERGLNGLYTHR
9   SERGLY

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