BMRB Entry 27461
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27461
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Title: Human TGIF1 c-term domain PubMed: 30060237
Deposition date: 2018-04-23 Original release date: 2018-07-17
Authors: Sunol Moreno, David; Macias, Maria J.
Citation: Guca, Ewelina; Sunol, David; Ruiz, Lidia; Konkol, Agnieszka; Cordero, Jorge; Torner, Carles; Aragon, Eric; Martin-Malpartida, Pau; Riera, Antoni; Macias, Maria. "TGIF1 homeodomain interacts with Smad MH1 domain and represses TGF-beta signaling." Nucleic Acids Res. 46, 9220-9235 (2018).
Assembly members:
TGIF1, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petM11
Entity Sequences (FASTA):
TGIF1: GAMGIKNFMPALEETPFHSC
TAGPNPTLGRPLSPKPSSPG
SVLARPSVICHTTVTALKDV
PFSLCQSVGVGQNTDIQQIA
AKNFTDTSLMYPED
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 149 |
| 15N chemical shifts | 78 |
| 1H chemical shifts | 78 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TGIF1 | 1 |
Entities:
Entity 1, TGIF1 94 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | ILE | LYS | ASN | PHE | MET | PRO | ||||
| 2 | ALA | LEU | GLU | GLU | THR | PRO | PHE | HIS | SER | CYS | ||||
| 3 | THR | ALA | GLY | PRO | ASN | PRO | THR | LEU | GLY | ARG | ||||
| 4 | PRO | LEU | SER | PRO | LYS | PRO | SER | SER | PRO | GLY | ||||
| 5 | SER | VAL | LEU | ALA | ARG | PRO | SER | VAL | ILE | CYS | ||||
| 6 | HIS | THR | THR | VAL | THR | ALA | LEU | LYS | ASP | VAL | ||||
| 7 | PRO | PHE | SER | LEU | CYS | GLN | SER | VAL | GLY | VAL | ||||
| 8 | GLY | GLN | ASN | THR | ASP | ILE | GLN | GLN | ILE | ALA | ||||
| 9 | ALA | LYS | ASN | PHE | THR | ASP | THR | SER | LEU | MET | ||||
| 10 | TYR | PRO | GLU | ASP |
Samples:
sample_1: TGIF1, [U-100% 13C; U-100% 15N], 100 mM; D2O, [U-2H], 10 % v/v; sodium phosphate 20 mM; sodium chloride 80 mM; TCEP 1 mM
sample_conditions_1: pH: 5.25; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts