BMRB Entry 27465
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27465
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Title: Sensory rhodopsin II has distinct fast internal motion and residual conformational entropy PubMed: 32277554
Deposition date: 2018-04-23 Original release date: 2020-04-07
Authors: O'Brien, Evan; Stetz, Matthew; Lin, Danny; Wand, Joshua
Citation: O'Brien, Evan; Fuglestad, Brian; Lessen, Henry; Stetz, Matthew; Lin, Danny; Marques, Bryan; Gupta, Kushol; Fleming, Karen; Wand, A Joshua. "Membrane Proteins Have Distinct Fast Internal Motion and Residual Conformational Entropy" Angew. Chem. Int. Ed. Engl. 59, 11108-11114 (2020).
Assembly members:
pSRII, polymer, 245 residues, Formula weight is not available
entity_RET, non-polymer, 284.436 Da.
Natural source: Common Name: Natronomonas pharaonis Taxonomy ID: 2257 Superkingdom: Archaea Kingdom: not available Genus/species: Natronomonas pharaonis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
pSRII: MVGLTTLFWLGAIGMLVGTL
AFAWAGRDAGSGERRYYVTL
VGISGIAAVAYVVMALGVGW
VPVAERTVFAPRYIDWILTT
PLIVYFLGLLAGLDSREFGI
VITLNTVVMLAGFAGAMVPG
IERYALFGMGAVAFLGLVYY
LVGPMTESASQRSSGIKSLY
VRLRNLTVILWAIYPFIWLL
GPPGVALLTPTVDVALIVYL
DLVTXVGFGFIALDAAATLR
AEHGESLAGVDTDAPAVADH
HHHHH
- order_parameters
| Data type | Count |
| order parameters | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pSRII | 1 |
| 2 | cofactor | 2 |
Entities:
Entity 1, pSRII 245 residues - Formula weight is not available
| 1 | MET | VAL | GLY | LEU | THR | THR | LEU | PHE | TRP | LEU | ||||
| 2 | GLY | ALA | ILE | GLY | MET | LEU | VAL | GLY | THR | LEU | ||||
| 3 | ALA | PHE | ALA | TRP | ALA | GLY | ARG | ASP | ALA | GLY | ||||
| 4 | SER | GLY | GLU | ARG | ARG | TYR | TYR | VAL | THR | LEU | ||||
| 5 | VAL | GLY | ILE | SER | GLY | ILE | ALA | ALA | VAL | ALA | ||||
| 6 | TYR | VAL | VAL | MET | ALA | LEU | GLY | VAL | GLY | TRP | ||||
| 7 | VAL | PRO | VAL | ALA | GLU | ARG | THR | VAL | PHE | ALA | ||||
| 8 | PRO | ARG | TYR | ILE | ASP | TRP | ILE | LEU | THR | THR | ||||
| 9 | PRO | LEU | ILE | VAL | TYR | PHE | LEU | GLY | LEU | LEU | ||||
| 10 | ALA | GLY | LEU | ASP | SER | ARG | GLU | PHE | GLY | ILE | ||||
| 11 | VAL | ILE | THR | LEU | ASN | THR | VAL | VAL | MET | LEU | ||||
| 12 | ALA | GLY | PHE | ALA | GLY | ALA | MET | VAL | PRO | GLY | ||||
| 13 | ILE | GLU | ARG | TYR | ALA | LEU | PHE | GLY | MET | GLY | ||||
| 14 | ALA | VAL | ALA | PHE | LEU | GLY | LEU | VAL | TYR | TYR | ||||
| 15 | LEU | VAL | GLY | PRO | MET | THR | GLU | SER | ALA | SER | ||||
| 16 | GLN | ARG | SER | SER | GLY | ILE | LYS | SER | LEU | TYR | ||||
| 17 | VAL | ARG | LEU | ARG | ASN | LEU | THR | VAL | ILE | LEU | ||||
| 18 | TRP | ALA | ILE | TYR | PRO | PHE | ILE | TRP | LEU | LEU | ||||
| 19 | GLY | PRO | PRO | GLY | VAL | ALA | LEU | LEU | THR | PRO | ||||
| 20 | THR | VAL | ASP | VAL | ALA | LEU | ILE | VAL | TYR | LEU | ||||
| 21 | ASP | LEU | VAL | THR | X | VAL | GLY | PHE | GLY | PHE | ||||
| 22 | ILE | ALA | LEU | ASP | ALA | ALA | ALA | THR | LEU | ARG | ||||
| 23 | ALA | GLU | HIS | GLY | GLU | SER | LEU | ALA | GLY | VAL | ||||
| 24 | ASP | THR | ASP | ALA | PRO | ALA | VAL | ALA | ASP | HIS | ||||
| 25 | HIS | HIS | HIS | HIS | HIS |
Entity 2, cofactor - C20 H28 O - 284.436 Da.
| 1 | RET |
Samples:
sample_1: pSRII, [U-100% 15N; U-75% 2H; U-100% 13CH3 ILVM], 0.4 mM; retinal 0.4 mM; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 3 mM; DHPC, [U-100% 2H aliphatic], 4 % w/v
sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 323 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| SQ 1H-13C CRLX | sample_1 | isotropic | sample_conditions_1 |
| MQ 1H-13C CRLX | sample_1 | isotropic | sample_conditions_1 |
| 15N TROSY T1 | sample_1 | isotropic | sample_conditions_1 |
| 15N TROSY T1rho | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 750 MHz
Related Database Links:
| PDB |