BMRB Entry 27471
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27471
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Title: peptide AapA1 toxin of Helicobacter pylori PubMed: 31476357
Deposition date: 2018-05-03 Original release date: 2020-02-28
Authors: Korkut, Dursun; Salgado, Gilmar
Citation: Korkut, Dursun Nizam; Alves, Isabel; Vogel, Alexander; Chabas, Sandrine; Sharma, Cynthia; Martinez, Denis; Loquet, Antoine; Salgado, Gilmar; Darfeuille, Fabien. "Structural insights into the AapA1 toxin of Helicobacter pylori" Biochim. Biophys. Acta Gen. Subj. 1864, 129423-129423 (2020).
Assembly members:
AapA1, polymer, 30 residues, 3435.22 Da.
Natural source: Common Name: Helicobacter pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
AapA1: MATKHGKNSWKTLYLKISFL
GCKVVVLLKR
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 31 |
| 1H chemical shifts | 222 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AapA1 | 1 |
Entities:
Entity 1, AapA1 30 residues - 3435.22 Da.
| 1 | MET | ALA | THR | LYS | HIS | GLY | LYS | ASN | SER | TRP | |
| 2 | LYS | THR | LEU | TYR | LEU | LYS | ILE | SER | PHE | LEU | |
| 3 | GLY | CYS | LYS | VAL | VAL | VAL | LEU | LEU | LYS | ARG |
Samples:
sample_1: AapA1 1.5 ± 5 mM; AapA1 0.2 ± 5 mM; TFE 40%; acetic acid 10 mM; NaCl 10 mM; DTT 2 mM
sample_conditions_1: ionic strength: 30 mM; pH: 4.7; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TopSpin_3.1 v3.1, Bruker Biospin, CCPN, Goddard - chemical shift assignment, collection, data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts