BMRB Entry 27484

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27484

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Title:
Backbone assignment of KSHV ORF57 68-178
Deposition date:
2018-05-15
Original release date:
2018-09-24
Authors:
Tunnicliffe, Richard; Tian, Xiaochen; Storer, Joanna; Sandri-Goldin, Rozanne; Golovanov, Alexander
Citation:

Citation: Tunnicliffe, Richard; Tian, Xiaochen; Storer, Joanna; Sandri-Goldin, Rozanne; Golovanov, Alexander. "Overlapping motifs on the herpes viral proteins ICP27 and ORF57 mediate interactions with the mRNA export adaptors ALYREF and UIF"  Sci. Rep. 8, 15005-15005 (2018).

Assembly members:

Assembly members:
ORF57, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human gammaherpesvirus 8   Taxonomy ID: 37296   Superkingdom: Viruses   Kingdom: not available   Genus/species: Rhadinovirus Human herpesvirus 8 type P

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ORF57: MGSSHHHHHHSSGLVPRGSH MGRIPKKSQGRSQLRSEIQF CSPLSRPRSPSPVNRYGKKI KFGTAGQNTRPPPEKRPRRR PRDRLQYGRTTRGGQCRAAP KRATRRPQVNCQRQDDDVRQ GVSDAVKKLRLP

Data sets:
Data typeCount
15N chemical shifts93
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ORF57 68-1781

Entities:

Entity 1, ORF57 68-178 132 residues - Formula weight is not available

N-terminal tag introdued in cloning is: MGSSHHHHHHSSGLVPRGSHM followed by KSHV ORF57 residues 68-178.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLYARGILEPROLYSLYSSERGLNGLY
4   ARGSERGLNLEUARGSERGLUILEGLNPHE
5   CYSSERPROLEUSERARGPROARGSERPRO
6   SERPROVALASNARGTYRGLYLYSLYSILE
7   LYSPHEGLYTHRALAGLYGLNASNTHRARG
8   PROPROPROGLULYSARGPROARGARGARG
9   PROARGASPARGLEUGLNTYRGLYARGTHR
10   THRARGGLYGLYGLNCYSARGALAALAPRO
11   LYSARGALATHRARGARGPROGLNVALASN
12   CYSGLNARGGLNASPASPASPVALARGGLN
13   GLYVALSERASPALAVALLYSLYSLEUARG
14   LEUPRO

Related Database Links:

UNP Q2HR75
AlphaFold O40938

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks