BMRB Entry 27488
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27488
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: ASK1-TBD PubMed: 31623019
Deposition date: 2018-05-22 Original release date: 2020-10-06
Authors: Psenakova, Katarina; Veverka, Vaclav
Citation: Psenakova, Katarina; Hexnerova, Rozalie; Srb, Pavel; Obsilova, Veronika; Veverka, Vaclav; Obsil, Tomas. "The redox-active site of thioredoxin is directly involved in apoptosis signal-regulating kinase 1 binding that is modulated by oxidative stress" FEBS J. 287, 1626-1644 (2020).
Assembly members:
ASK1-TBD, polymer, 185 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: N/A
Entity Sequences (FASTA):
ASK1-TBD: GAMGSSRRTTVAYVINEASQ
GQLVVAESEALQSLREACET
VGATLETLHFGKLDFGETTV
LDRFYNADIAVVEMSDAFRQ
PSLFYHLGVRESFSMANNII
LYCDTNSDSLQSLKEIICQK
NTMCTGNYTFVPYMITPHNK
VYCCDSSFMKGLTELMQPNF
ELLLGPICLPLVDRFIQLLK
VAQAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 374 |
| 15N chemical shifts | 120 |
| 1H chemical shifts | 468 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ASK1-TBD | 1 |
Entities:
Entity 1, ASK1-TBD 185 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | SER | SER | ARG | ARG | THR | THR | ||||
| 2 | VAL | ALA | TYR | VAL | ILE | ASN | GLU | ALA | SER | GLN | ||||
| 3 | GLY | GLN | LEU | VAL | VAL | ALA | GLU | SER | GLU | ALA | ||||
| 4 | LEU | GLN | SER | LEU | ARG | GLU | ALA | CYS | GLU | THR | ||||
| 5 | VAL | GLY | ALA | THR | LEU | GLU | THR | LEU | HIS | PHE | ||||
| 6 | GLY | LYS | LEU | ASP | PHE | GLY | GLU | THR | THR | VAL | ||||
| 7 | LEU | ASP | ARG | PHE | TYR | ASN | ALA | ASP | ILE | ALA | ||||
| 8 | VAL | VAL | GLU | MET | SER | ASP | ALA | PHE | ARG | GLN | ||||
| 9 | PRO | SER | LEU | PHE | TYR | HIS | LEU | GLY | VAL | ARG | ||||
| 10 | GLU | SER | PHE | SER | MET | ALA | ASN | ASN | ILE | ILE | ||||
| 11 | LEU | TYR | CYS | ASP | THR | ASN | SER | ASP | SER | LEU | ||||
| 12 | GLN | SER | LEU | LYS | GLU | ILE | ILE | CYS | GLN | LYS | ||||
| 13 | ASN | THR | MET | CYS | THR | GLY | ASN | TYR | THR | PHE | ||||
| 14 | VAL | PRO | TYR | MET | ILE | THR | PRO | HIS | ASN | LYS | ||||
| 15 | VAL | TYR | CYS | CYS | ASP | SER | SER | PHE | MET | LYS | ||||
| 16 | GLY | LEU | THR | GLU | LEU | MET | GLN | PRO | ASN | PHE | ||||
| 17 | GLU | LEU | LEU | LEU | GLY | PRO | ILE | CYS | LEU | PRO | ||||
| 18 | LEU | VAL | ASP | ARG | PHE | ILE | GLN | LEU | LEU | LYS | ||||
| 19 | VAL | ALA | GLN | ALA | SER |
Samples:
sample_1: ASK1-TBD, [U-100% 13C; U-100% 15N; U-80% 2H], 300 uM
sample_conditions_1: ionic strength: 0.2 M; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts