BMRB Entry 27530
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27530
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Title: apoCaM bound to Cav1.2 IQ
Deposition date: 2018-06-28 Original release date: 2019-09-17
Authors: Turner, Matthew; Ames, James; Anderson, David
Citation: Turner, Matthew; Ames, James; Anderson, David. "ApoCaM bound to Cav1.2 IQ" Cell ., .-..
Assembly members:
apoCaM, polymer, 149 residues, Formula weight is not available
Natural source: Common Name: African clawed frog Taxonomy ID: 8355 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus laevis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 3b
Entity Sequences (FASTA):
apoCaM: MADQLTEEQIAEFKEAFSLF
DKDGDGTITTKELGTVMRSL
GQNPTEAELQDMINEVDADG
NGTIDFPEFLTMMARKMKDT
DSEEEIREAFRVFDKDGNGY
ISAAELRHVMTNLGEKLTDE
EVDEMIREADIDGDGQVNYE
EFVQMMTAK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 115 |
| 1H chemical shifts | 115 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | apoCaM | 1 |
Entities:
Entity 1, apoCaM 149 residues - Formula weight is not available
| 1 | MET | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ||||
| 2 | ALA | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ||||
| 3 | ASP | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | ||||
| 4 | LYS | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | ||||
| 5 | GLY | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ||||
| 6 | ASP | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ||||
| 7 | ASN | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | ||||
| 8 | THR | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ||||
| 9 | ASP | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ||||
| 10 | ARG | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ||||
| 11 | ILE | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | ||||
| 12 | THR | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | ||||
| 13 | GLU | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ||||
| 14 | ILE | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | ||||
| 15 | GLU | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Samples:
sample_1: apoCaM, [U-100% 15N], 350 uM; apoCaM, [U-100% 13C; U-100% 15N], 350 uM
sample_conditions_1: ionic strength: 0.150 M; pH: 7.3; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts