BMRB Entry 27545
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27545
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Title: Pongo abelii ribonuclease 3 backbone assignment
Deposition date: 2018-07-13 Original release date: 2018-07-19
Authors: Bernard, David; Doucet, Nicolas
Citation: Bernard, David; Narayanan, Chitra; Bafna, Khushboo; Bhojane, Purva; Letourneau, Myriam; Howell, Elizabeth; Agarwal, Pratul; Doucet, Nicolas. "Pongo abelii ribonuclease 3 backbone assignment" .
Assembly members:
PaRNase3, polymer, 133 residues, 15188 Da.
Natural source: Common Name: Sumatran orangutan Taxonomy ID: 9601 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Pongo abelii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress 414
Entity Sequences (FASTA):
PaRNase3: KPRQFTRAQWFAIQHVSLNP
PQCATAMRVINNYQRRCKDQ
NTFLRTTFANVVNVCGNPNI
TCPSNRSRNNCHHSGVQVPF
IYCNLTTPSPQNISNCSYAN
ITGRRFYIVACDNRDPRDSP
QYPVVPVHLDTII
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 248 |
| 15N chemical shifts | 118 |
| 1H chemical shifts | 120 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pongo abelii RNase 3 | 1 |
Entities:
Entity 1, Pongo abelii RNase 3 133 residues - 15188 Da.
| 1 | LYS | PRO | ARG | GLN | PHE | THR | ARG | ALA | GLN | TRP | ||||
| 2 | PHE | ALA | ILE | GLN | HIS | VAL | SER | LEU | ASN | PRO | ||||
| 3 | PRO | GLN | CYS | ALA | THR | ALA | MET | ARG | VAL | ILE | ||||
| 4 | ASN | ASN | TYR | GLN | ARG | ARG | CYS | LYS | ASP | GLN | ||||
| 5 | ASN | THR | PHE | LEU | ARG | THR | THR | PHE | ALA | ASN | ||||
| 6 | VAL | VAL | ASN | VAL | CYS | GLY | ASN | PRO | ASN | ILE | ||||
| 7 | THR | CYS | PRO | SER | ASN | ARG | SER | ARG | ASN | ASN | ||||
| 8 | CYS | HIS | HIS | SER | GLY | VAL | GLN | VAL | PRO | PHE | ||||
| 9 | ILE | TYR | CYS | ASN | LEU | THR | THR | PRO | SER | PRO | ||||
| 10 | GLN | ASN | ILE | SER | ASN | CYS | SER | TYR | ALA | ASN | ||||
| 11 | ILE | THR | GLY | ARG | ARG | PHE | TYR | ILE | VAL | ALA | ||||
| 12 | CYS | ASP | ASN | ARG | ASP | PRO | ARG | ASP | SER | PRO | ||||
| 13 | GLN | TYR | PRO | VAL | VAL | PRO | VAL | HIS | LEU | ASP | ||||
| 14 | THR | ILE | ILE |
Samples:
sample_1: PaRNase3, [U-99% 13C; U-99% 15N], 300 uM; sodium acetate 15 mM
sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr v2.4, CCPN - peak picking
NMR spectrometers:
- Agilent INOVA 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts