BMRB Entry 27546
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27546
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Title: Aotus trivirgatus Eosinophil-Derived Neurotoxin backbone assignment
Deposition date: 2018-07-13 Original release date: 2018-07-19
Authors: Bernard, David; Doucet, Nicolas
Citation: Bernard, David; Narayanan, Chitra; Bafna, Khushboo; Bhojane, Purva; Letourneau, Myriam; Howell, Elizabeth; Agarwal, Pratul; Doucet, Nicolas. "Aotus trivirgatus Eosinophil-Derived Neurotoxin backbone assignment" .
Assembly members:
AtEDN, polymer, 131 residues, 14857 Da.
Natural source: Common Name: douroucouli Taxonomy ID: 9505 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Aotus trivirgatus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJexpress 414
Entity Sequences (FASTA):
AtEDN: APQKFTRAQWFSIQHIQTTP
LRCTNAMRAINKYQHRCKNQ
NTFLHTTFAAVVNVCGNTNI
TCPRNASLNNCHHSRVQVPL
TYCNLTGPPTITNCVYSSTQ
ANMFYVVACDNRDQRDPPQY
PVVPVHLDTTI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 254 |
| 15N chemical shifts | 119 |
| 1H chemical shifts | 119 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Aotus trivirgatus EDN | 1 |
Entities:
Entity 1, Aotus trivirgatus EDN 131 residues - 14857 Da.
| 1 | ALA | PRO | GLN | LYS | PHE | THR | ARG | ALA | GLN | TRP | ||||
| 2 | PHE | SER | ILE | GLN | HIS | ILE | GLN | THR | THR | PRO | ||||
| 3 | LEU | ARG | CYS | THR | ASN | ALA | MET | ARG | ALA | ILE | ||||
| 4 | ASN | LYS | TYR | GLN | HIS | ARG | CYS | LYS | ASN | GLN | ||||
| 5 | ASN | THR | PHE | LEU | HIS | THR | THR | PHE | ALA | ALA | ||||
| 6 | VAL | VAL | ASN | VAL | CYS | GLY | ASN | THR | ASN | ILE | ||||
| 7 | THR | CYS | PRO | ARG | ASN | ALA | SER | LEU | ASN | ASN | ||||
| 8 | CYS | HIS | HIS | SER | ARG | VAL | GLN | VAL | PRO | LEU | ||||
| 9 | THR | TYR | CYS | ASN | LEU | THR | GLY | PRO | PRO | THR | ||||
| 10 | ILE | THR | ASN | CYS | VAL | TYR | SER | SER | THR | GLN | ||||
| 11 | ALA | ASN | MET | PHE | TYR | VAL | VAL | ALA | CYS | ASP | ||||
| 12 | ASN | ARG | ASP | GLN | ARG | ASP | PRO | PRO | GLN | TYR | ||||
| 13 | PRO | VAL | VAL | PRO | VAL | HIS | LEU | ASP | THR | THR | ||||
| 14 | ILE |
Samples:
sample_1: AtEDN, [U-99% 13C; U-99% 15N], 300 uM; sodium acetate 15 mM
sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr v2.4, CCPN - peak picking
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts